海南部分荔枝种质的RAPD分析

RAPD Analysis of Genetic Relationship among Partial Litchi Germplasms in Hainan Island

采用10个10碱基的随机引物,通过RAPD分析的方法,在分子水平上对海南30份荔枝种质进行鉴定、分类和亲缘关系分析。结果表明,大多数种质相似系数在55％-80％之间,说明他们之间的亲缘关系较近;在相似系数为0.61的水平上,30份海南荔枝种质被分为4组,与以龟裂片特征为主要分类依据的形态分类有一定的相关性,但是RAPD分类比形态分类的分类范围更细。因此,传统形态分类不能完全反映出品种间的亲缘关系,有待结合RAPD分析方法进一步完善。

Identification, classification and genetic relationship of 30 litchi germplasms of Hainan island were measured at the molecular level by random amplified polymorphic DNA ( RAPD) with ten arbitrary primers of 10 bases. The results revealed that similarity coefficients of most of litchi germplasms were among 55% -80% , which indicated that the genetic relationships between them were closer related. 30 litchi germ-plasms were divided into 4 groups at the level of the similarity coefficient of 61%. Although a certain correlation with the morphological classification based mainly on the characteristic of tubercles on the ripe fruit skin, the range of classification by RAPD was more detailed than that of morphological classification. It is concluded that the traditional morphological classification system can not reflect fully the genetic relationships between litchi germplasms, and need further improvement according to the result by RAPD analysis.