外源乙烯对草莓果实微粒体Ca^(2+)-ATPase活性和膜脂过氧化的调节

Regulation of Exogenous Ethylene on Ca^(2+)-ATPase Activity and Lipid Perox-idation of Microsomal Membrane in Strawberry Fruits

以乳白期‘春星’草莓(Fragaria ananassa Duch.‘Chunxing’)果实为试材,研究了经钙调素(CaM)拮抗剂三氟拉嗪(TFP)、氯丙嗪(CPZ)各100μmol·L-1和钙通道阻塞剂异博定(Verapamil)100μmol·L-1预处理后再用乙烯(50μL·L-1)处理的草莓果实中微粒体Ca2+-ATP酶活性、O2·-产生速率和MDA含量的变化。结果表明,外源乙烯对微粒体膜O2·-产生速率无显著影响,处理早期提高微粒体膜Ca2+-ATPase总活性,对MDA含量影响不大;后期加速微粒体膜Ca2+-ATPase总活性下降,但仍保持较高的MDA含量和线粒体膜Ca2+-ATPase活性。CPZ、TFP和Verapamil预处理降低了上述乙烯处理下Ca2+-ATPase活性和MDA含量,但对微粒体膜O2·-产生速率亦无显著影响,这说明细胞内Ca2+和CaM可能参与了乙烯诱导的膜Ca2+-ATP酶活性与膜脂过氧化水平的调节。

The strawberry fruits (Fragaria ananassa Duch. 'Chunxing' ) harvested at white stage were used to investigate the effects of exogenous ethylene (50 μL · L -1 ) on Ca -ATPase activities, O2-production rate and MDA content of microsomal membrane with or without pretreatment of 100 μmol · L-1 chloropromaize ( CPZ) , trifluoperizine ( TFP) and verapamil ( VER) , respectively. The results showed that the exogenous ethylene had no obvious influence on O2- production rate, and had no much effect on MDA content, but stimulated Ca2+-ATPase activity of microsomal membrane at 12 h after ethylene treatment. Whereas a decreasing of Ca2 + -ATPase activities, and relatively higher MDA content were found at 24 h after ethylene removal. The pretreatment of CPZ, TFP and VER reduced the Ca2 + -ATPase activities and MDA coiitent of microsomal membrane under ethylene treatment, but had no significant effect on O2- production rate. These suggested that cellular Ca + -CaM may be involved in regulation of Ca + -ATPase activity and lipid peroxidation of microsomal membrane induced by ethylene.