人皮肤T细胞淋巴瘤系Hut-78细胞FHIT基因缺失的研究

The Study of the Loss of FHIT Gene in Cutaneous T Cell Lymphomas Hut-78 Cell

目的 探讨抑癌基因FHIT在Hut 78细胞中的缺失情况。方法 用巢式RT PCR方法研究FHIT的缺失情况。结果 Hut 78细胞中FHIT基因mRNA比正常缩短 ,外显子 5丢失。结论 FHIT基因外显子 5的缺失可能是皮肤T细胞淋巴瘤FHIT基因失活的主要方式 。

ObjectiveTo investigate the loss patterns of FHIT gene,a tumor suppressor gene,in the Hut-78 cells.MethodsNested RT-PCR was used to amplify the whole leng th of mRNA.ResultsThe mRNA of FHIT gene declined signifi cantly in Hut-78 cell in comparison with the normal lymphocytes,exon-5 of FHIT gene was lost. ConclusionThe loss of exon-5 may be one of the major in activation patterns of FHIT gene in cutaneous T cell lymphomas,which may have v ery important role in the pathogenesis of cutaneous T cell lymphomas.