肺炎链球菌诱发小鼠细菌性鼻腔鼻窦炎模型

Studies of a Mouse Model of Acute Bacterial Rhinosinusitis by Inoculation with Streptococcus pneumoniae

目的 探讨昆明小鼠急性鼻腔鼻窦炎动物模型的制作方法。方法 清洁级昆明小鼠 4 0只 ,35只鼻腔接种 3型荚膜型肺炎链球菌使动物感染 ,5只鼻腔滴入灭菌生理盐水作对照。实验组分别于接种后第 2、5、8、11、14、2 1、2 8d处死 ,每个时点处死 5只。取鼻面骨 ,石蜡包埋 ,切片 ,HE染色 ,观察动物鼻腔、鼻窦炎细胞浸润情况。结果 接种后第 2、5天动物鼻腔鼻窦出现红细胞渗出 ,第 8天开始观察到中性粒细胞集落形成 ,第 11、14天达到高峰 ,2 1天已明显消退 ,第 2 8天基本恢复正常。对照组无炎症表现。结论 采用 3型肺炎链球菌成功诱发了昆明小鼠的急性细菌性鼻腔鼻窦炎。可作为临床鼻腔鼻窦炎症研究的动物模型。

Objective To develop a mouse model of acute bacterial rhinosinusitis. Method 35 Swiss mice were inoculated intranasally with type 3 encapsuled Streptococcus pneumoniae solution. The controls (5 Swiss mice) were inoculated with sterilized saline. The histological sections of the nasal cavities and nasal sinuses were stained with hematoxylin-eosin. Neutrophil clusters in the nasal cavities and nasal sinuses were checked. Results There was significant red blood cells exudation before the 5th day of inoculation. Then on day 8, neutrophil clusters were examined and by day 11 and 14 the number of neutrophil clusters gained its peak. The amount of inflammation disappeared by day 28. Conclusions Streptococcus pneumoniae induces acute bacterial rhinosinusitis in Swiss mouse, and it can be used as an animal model of acute bacterial rhinosinusitis.