3个不同细胞株用于垫料毒性实验的效果比较

The Effect Comparison of Three Cell Lines on Cytotoxicity Experiment of Bedding Materials

目的 选择一种比较敏感的细胞用以进行垫料物质的细胞毒性研究。方法 将不同浓度的垫料物质丙酮提取物分别加入体外培养的小鼠成纤维细胞、小鼠H2 2 H2 D8细胞株和小鼠S1 80 _S2 D9细胞 ,孵育 72h后用考马斯亮蓝法测定总蛋白 ,计算细胞存活率 ,比较 3种细胞对不同垫料丙酮提取物的细胞毒性反应。结果 在 1～ 4 0mg/ml浓度范围 ,H2 2 _H2 D8对各种垫料物质细胞毒性反应最敏感。结论 H2 2 _H2 D8细胞可作为垫料物质细胞毒性研究的细胞株。

Objective To find a sort of cells which is more sensitive for the cytotoxicity experiment on bedding materials.Methods The acetone extractions of beddings with different concentration were added in mouse fibroblast,S 180_S-2D-9 cells and H 22_H-2D-8 cells.The total protein of cells was measured by the method of Comassie brilliant blue after being cultured 72 hours and the livability of cells was counted.Then, the sensitivity of the three cells was compared.Results The H 22_H-2D-8 cells were more sensitive in the range of 1-40 mg/ml.Conclusion H 22_H-2D-8 cells can be used in the cytotoxicity experiment on bedding materials.