Zmu-1:DHP豚鼠的随机扩增多态性DNA分析

The RAPD Analysis to Zmu-1:DHP Guinea Pig

目的 研究Zmu 1:DHP豚鼠基因组DNA的遗传多态性及其遗传概貌 ,探讨随机引物PCR(RAPD PCR)在豚鼠遗传质量检测中运用的可行性。方法 用RAPD PCR法 ,对新培育的Zmu 1:DHP豚鼠和对照品系DHP豚鼠的基因组DNA进行PCR扩增。结果 从 4 0条随机引物中 ,筛选出 2条呈多态性的引物 ,分别是第S1 2 0 2 号和第S1 2 1 9号引物。Zmu 1:DHP品系的扩增产物无多态性变化 ,而且其条带与多数DHP品系的个体相同。少数DHP品系的个体呈多态性变化 ,并找到该品系增加和缺失的特征性条带各一种。结论 Zmu 1:DHP品系的基因纯合性及个体一致性较DHP品系高。二个品系的DNA序列存在较少差异 ,说明豚鼠品系间遗传结构比较接近。一旦选定具有多态性的引物 ,RAPD PCR法可以区分豚鼠品系 ,适用于豚鼠遗传质量检定。

Objective To study the genetic polymorphism of genomic DNA and genetic profile in a new guinea pig strain,and probe into the feasibility of RAPD-PCR method in genetic monitoring in guinea pig. Method The genomic DNA of Zmu-1:DHP strain and DHP guinea pig,as a compared strain, are amplified with the RAPD-PCR method. Results Two random primers with polymorphism were screened from 40 primers. No polymorphism occured in the amplified DNA product of Zmu-1:DHP strain, and the pattern of amplified product of Zmu-1:DHP strain was identical with most individuals of DHP strain. The amplified product of a small number of individuals in DHP strain demonstrated the polymorphism, and two characteristic DNA fragments were found in the strain.Conclusion The genetic homogeneity and the individual identity of Zmu-1:DHP strain is higher than DHP strain. There is a little difference between DNA sequences of the both strains. RAPD-PCR could be used in the identity of the strains and the monitoring of the genetic quality in guinea pig.