四氯化碳对非洲绿猴肾细胞损伤机理的研究

Study on the Injury Mechanism of Carbon Tetrachloride to Vero Cells

目的 实验研究四氯化碳染毒 2 4h对非洲绿猴肾细胞的损伤及其机理。方法 运用显微荧光术测定非洲绿猴肾细胞 (Vero细胞 )内活性氧 (ROS)含量及游离Ca2 + 浓度 ,同时 ,测定Vero细胞培养上清液乳酸脱氢酶(LDH)活力用于检查Vero细胞受损情况。结果 接触浓度为 1 0mmol L四氯化碳的Vero细胞内ROS含量及游离Ca2 + 浓度与对照组比较差异无显著性 (P >0 .0 5 ) ,同时 ,表示Vero细胞受损指标 (LDH活力 )差异也无显著性 (P >0 .0 5 ) ;而接触浓度为 4 0、8.0mmol L四氯化碳的Vero细胞内ROS含量及游离Ca2 + 浓度均显著性高于对照组 (P <0 .0 1) ,其Vero细胞受损也均显著性增加 (P <0 .0 1)。结论 较高浓度的四氯化碳能损伤Vero细胞 ,其损伤的可能途径是通过提高Vero细胞内ROS含量及游离Ca2 + 浓度。

Objective To understand the injury mechanism of Vero cells (African green monkey kidney cells)exposing to carbon tetrachloride (CCl\-4) for 24 hours. Methods\ The content of reactive oxygen species (ROS) and cytoplasmic Ca 2+ concentration in Vero cells were measured with microfluorometry.Meanwhile,the injury of Vero cells was detected with lactate dehydrogenase (LDH) activity in the supernate of the medium for Vero cells.Results\ There were no any differences for the above indices between the group at 2.0mmol/L concentration CCl\-4 and control group statistically (\%P\%>0.05).In the other hand,both the content of reactive oxygen species and cytoplasmic Ca 2+ concentration in the Vero cells exposing to CCl\-4 at 4.0mmol/L and 8.0mmol/L concentration were higher than those in the control group ( P <0.01) respectively, and the activity of LDH in supernate of the medium for Vero cells was higher than that of latter ( P <0.01).Conclusion\ The possible injury mechanisms of Vero cells exposing to CCl\-4 of high concentration are partly mediated by increasing the content of reactive oxygen species and cytoplasmic Ca 2+ concentration in Vero cells.