摘要
AIM: To explore the potential carcinogenicity of bile from congenital choledochal cyst (CCC) patients and the mechanism of the carcinogenesis in congenital choledochal cyst patients.METHODS: 20 bile samples from congenital choledochalcyst patients and 10 normal control bile samples were usedfor this study. The proliferative effect of bile was measuredby using Methabenzthiazuron (MTT) assay; Cell cycle andapoptosis were analyzed by using flow cytometry (FCM),and the PGE2 levels in the supernatant of culturedcholangiocarcinoma cells were quantitated by enzyme-linkedimmunoabsordent assay (ELISA).RESULTS: CCC bile could significantly promote theproliferation of human cholangiocarcinoma QBC939 cellscompared with normal bile (P＝0.001) and negative controlgroup (P＝0.002), and the proliferative effect of CCC bilecould be abolished by addition of cyclooxygenase-2 specificinhibitor celecoxib (20 μM). The QBC939 cells proliferativeindex was increased significantly after treated with 1% bilefrom CCC patient (P＝0.008) for 24 h, the percentage of Sphase (29.48±3.27)% was increased remarkably (P＜0.001)compared with normal bile (11.72±2.70) %, and thepercentage of G0/G1 phase (54.19±9.46) % was decreasedremarkably (P＝0.042) compared with normal bile (69.16±10.88) %, however, bile from CCC patient had no significantinfluence on apoptosis of QBC939 cells (P＝0.719).CONCLUSION: Bile from congenital choledochal cyst patientscan promote the proliferation of human cholangiocarcinomaQBC939 cells via COX-2 and PGE2 pathway.
AIM:To explore the potential carcinogenicity of bile from congenital choledochal cyst(CCC)patients and the mechanism of the carcinogenesis in congenital choledochal cyst patients. METHODS:20 bile samples from congenital choledochal cyst patients and 10 normal control bile samples were used for this study.The proliferative effect of bile was measured by using Methabenzthiazuron(MTT)assay;Cell cycle and apoptosis were analyzed by using flow cytometry(FCM), and the PGE_2 levels in the supernatant of cultured cholangiocarcinoma cells were quantitated by enzyme-linked immunoabsordent assay(ELISA). RESULTS:CCC bile could significantly promote the proliferation of human cholangiocarcinoma QBC939 cells compared with normal bile(P=0.001)and negative control group(P=0.002),and the proliferative effect of CCC bile could be abolished by addition of cyclooxygenase-2 specific inhibitor celecoxib(20 μM).The QBC939 cells proliferative index was increased significantly after treated with 1%bile from CCC patient(P=0.008)for 24 h,the percentage of S phase(29.48±3.27)%was increased remarkably(P<0.001) compared with normal bile(11.72±2.70)%,and the percentage of G0/G1 phase(54.19±9.46)%was decreased remarkably(P=0.042)compared with normal bile(69.16± 10.88)%,however,bile from CCC patient had no significant influence on apoptosis of QBC939 cells(P=0.719). CONCLUSION:Bile from congenital choledochal cyst patients can promote the proliferation of human cholangiocarcinoma QBC939 cells via COX-2 and PGE_2 pathway.
