摘要
AIM: To study the effects of pentoxifylline (PTX) on thecontent of hepatic TGF-β1, type Ⅰ and type Ⅲ collagen inschistosomiasis japonica mice with liver fibrosis and itsmechanism of anti-fibrosis.METHODS: Forty mice with schistosomiasis were dividedinto four groups: one group as control without anytreatment, other three were treated with Praziquantel 500mg/(kg.d)for 2 d, high dose PTX 360 mg/(kg.d) for 8 wk,and low dose PTX 180 mg/(kg.d) for 8 wk respectively.Immunohistochemical technique and multimedia colorpathographic analysis system were applied to observe thecontent change of hepatic TGF-β1, type Ⅰ and type Ⅲcollagen in schistosomiasis japonica mice with liver fibrosisbefore and after PTX treatment.RESULTS: Effects of PTX on the content change of hepaticTGF-β1, type Ⅰ and type Ⅲ collagen in schistosomiasis japonicamice with liver fibrosis were related to the dosage of PTX,high dose PTX treated group could significantly reduce thecontent of TGF-β1 (0.709±0.111), type Ⅰ (0.644±0.108) andtype Ⅲ (0.654±0.152) collagen compared with those ofcontrol group (0.883±0.140, 0.771±0.156, 0.822±0.129)with statistical significance (P＜0.05). Low dose PTX couldalso reduce the hepatic content of TGF-β1 (0.752±0.152),type Ⅰ (0.733±0.117) and type Ⅲ (0.788±0.147) collagen,but without statistical significance (P＞0.05). Both high doseand low dose PTX groups have significant differences onthe content of TGF-β1, type Ⅰ and type Ⅲ collagen (P＜0.05,P＜0.05, P＜ 0.01,respectively).CONCLUSION: High dose of PTX treatment could reducethe content of hepatic TGF-β1, type Ⅰ and type Ⅲ collagensignificantly in schistosomiasis japonica mice with liverfibrosis, and thus plays its role of antifibrosis.
AIM:To study the effects of pentoxifylline(PTX)on the content of hepatic TGF-β1,type Ⅰ and type Ⅲ collagen in schistosomiasis japonica mice with liver fibrosis and its mechanism of anti-fibrosis. METHODS:Forty mice with schistosomiasis were divided into four groups:one group as control without any treatment,other three were treated with Praziquantel 500 mg/(kg·d)for 2 d,high dose PTX 360 mg/(kg·d)for 8 wk, and low dose PTX 180 mg/(kg.d)for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content change of hepatic TGF-β1,type Ⅰ and type Ⅲ collagen in schistosomiasis japonica mice with liver fibrosis before and after PTX treatment. RESULTS:Effects of PTX on the content change of hepatic TGF-β1,type Ⅰ and type Ⅲ collagen in schistosomiasis japonica mice with liver fibrosis were related to the dosage of PTX, high dose PTX treated group could significantly reduce the content of TGF-β1(0.709±0.111),type Ⅰ(0.644±0.108)and type Ⅲ(0.654±0.152)collagen compared with those of control group(0.883±0.140,0.771±0.156,0.822±0.129) with statistical significance(P<0.05).Low dose PTX could also reduce the hepatic content of TGF-β1(0.752±0.152), type Ⅰ(0.733±0.117)and type Ⅲ(0.788±0.147)collagen, but without statistical significance(P>0.05).Both high dose and low dose PTX groups have significant differences on the content of TGF-β1,type Ⅰ and type Ⅲ collagen(P<0.05, P<0.05,P<0.01,respectively). CONCLUSION:High dose of PTX treatment could reduce the content of hepatic TGF-β1,type Ⅰ and type Ⅲ collagen significantly in schistosomiasis japonica mice with liver fibrosis,and thus plays its role of antifibrosis.
基金
the Science Research Foundation of Schistosomiasis of Hubei Province,No.2000
