摘要
AIM: To study the expression of cyclooxygenase-2 (COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS: With reference to the expression of β-actin gene,COX-2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients bysemiquantitative reverse transcription- polymerase chainreaction (RT-PCR). Quantitation of relative band Adj volumecounts was performed using molecular Analyst for windowssoftware. The COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin.RESULTS: The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa (0.5966±0.2659vs 0.2979+0.171, u=5.4309, P＜0.01). Significantly higherexpression of COX-2 mRNA was also observed in patientswith lymph node involvement than that in those without(0.6775±0.2486 vs 0.4105±0.2182, t=2.9341, P＜0.01).Furthermore, the staging in the UICC TNM classificationsignificantly correlated with COX-2 overexpression (F＝3.656,P＜0.05), the COX-2 index in stage Ⅲ and IV was significantlyhigher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3.4906, P＜0.05). The COX-2 index showed no correlationwith patient's age, sex, blood group, tumor location, grosstyping, depth of invasion, differentiation, and the greatesttumor dimension (P＞0.05).CONCLUSION: Expression of COX-2 mRNA in gastriccarcinoma was significantly higher, which may enhancelymphatic metastasis in patients with gastric carcinoma. Thestaging in the UICC TNM classification was significantlycorrelated with COX-2 over-expression. COX-2 may contributeto progression of tumor in human gastric adenocarcinoma.
AIM:To study the expression of cyclooxygenase-2(COX-2) gene in gastric cancer and the relationship between COX-2 expression and clinicopathologic features of gastric cancer. METHODS:With reference to the expression of β-actin gene, COX-2 mRNA level was examined in cancerous tissues and adjacent noncancerous mucosa from 33 patients by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR).Quantitation of relative band Adj volume counts was performed using molecular Analyst for windows software.The COX-2 index was determined from the band Adj volume counts ratio of COX-2 to constitutively expressed actin. RESULTS:The COX-2 index in gastric carcinoma was significantly higher than that in normal mucosa(0.5966±0.2659 vs 0.2979±0.171,u=5.4309,P<0.01).Significantly higher expression of COX-2 mRNA was also observed in patients with lymph node involvement than that in those without (0.6775±0.2486 vs 0.4105±0.2182,t=2.9341,P<0.01). Furthermore,the staging in the UICC TNM classification significantly correlated with COX-2 overexpression(F=3.656, P<0.05),the COX-2 index in stage Ⅲ and Ⅳ was significantly higher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3. 4906,P<0.05).The COX-2 index showed no correlation with patient's age,sex,blood group,tumor location,gross typing,depth of invasion,differentiation,and the greatest tumor dimension(P>0.05). CONCLUSION:Expression of COX-2 mRNA in gastric carcinoma was significantly higher,which may enhance lymphatic metastasis in patients with gastric carcinoma.The staging in the UICC TNM classification was significantly correlated with COX-2 over-expression.COX-2 may contribute to progression of tumor in human gastric adenocarcinoma.
