摘要
AIM: To investigate the role of tri-iodothyronine supplementin protecting gut barrier in septic rats.METHODS: Twenty-two rats were randomized into threegroups: sham group (n=6), sepsis group (n=8), and sepsisplus tri-iodothyronine (T3) group (n=8). Septic rat modelwas established through cecal ligation and puncture (CLP).After 5 h, sham and sepsis groups received saline, and theremaining group received T3 intraperitoneally. Twenty-onehrs After CLP, intestinal permeability and serum free T3 andT4 were measured with fluorescence spectrophotometer andby radioimmunoassay, respectively. Intestinal ultrastructureand histologic morphology were observed under transmissionelectron microscopy (TEM) and light microscopy, respectively.RESULTS: After 21 h, septic symptoms and signs in sepsisplus T3 group were milder than those in sepsis group. SerumFT3 or FT4 concentration in sepsis group was lower than thatin sham group (1.59±0.20, 3.41±2.14 pmol/L vs 3.44±1.40,9.53+3.39 pmol/L, P＜0.05), and FT3 concentration in sepsisplus T3 group (3.40±1.65 pmol/L, P＜0.05) was corrected.Portal concentration of fluorescein isothiocyanate-dextran(FITC-D) in sepsis group (2.51±0.56 mg/L) was higher thanthat in sham group (1.22±0.21 mg/L) (P＜0.01), and in sepsisplus T3group (1.68+0.38 mg/L) it was decreased significantly(P＜0.01). TEM and light microscopy showed that T3supplement preserved well ultrastructure and morphologyof intetinal mucosa in septic rats.CONCLUSION: Tri-iodothyronine supplement protects gutbarrier in septic rats.
AIM:To investigate the role of tri-iodothyronine supplement in protecting gut barrier in septic rats. METHODS:Twenty-two rats were randomized into three groups:sham group(n=6),sepsis group(n=8),and sepsis plus tri-iodothyronine(T_3)group(n=8).Septic rat model was established through cecal ligation and puncture(CLP). After 5 h,sham and sepsis groups received saline,and the remaining group received T_3 intraperitoneally.Twenty-one hrs After CLP,intestinal permeability and serum free T_3 and T4 were measured with fluorescence Spectrophotometer and by radioimmunoassay,respectively.Intestinal ultrastructure and histologic morphology were observed under transmission electron microscopy(TEM)and light microscopy,respectively. RESULTS:After 21 h,septic symptoms and signs in sepsis plus T_3 group were milder than those in sepsis group.Serum FT_3 or FT_4 concentration in sepsis group was lower than that in sham group(1.59±0.20,3.41±2.14 pmol/L vs3.44±1.40, 9.53±3.39 pmol/L,P<0.05),and FT_3 concentration in sepsis plus T_3 group(3.40±1.65 pmol/L,P<0.05)was corrected. Portal concentration of fluorescein isothiocyanate-dextran (FITC-D)in sepsis group(2.51±0.56 mg/L)was higher than that in sham group(1.22±0.21 mg/L)(P<0.01),and in sepsis plus T_3 group(1.68±0.38 mg/L)it was decreased significantly (P<0.01).TEM and light microscopy showed that T_3 supplement preserved well ultrastructure and morphology of intetinal mucosa in septic rats. CONCLUSION:Tri-iodothyronine supplement protects gut barrier in septic rats.
基金
the Scientific Research Fund of Chinese Ministry of Health,No.98-1-112
