摘要
目的研究卵巢上皮性癌细胞核DNA含量及其与卵巢癌化疗和预后的关系。方法应用自动图像分析系统对 2 5例术前未化疗及 2 0例术前化疗的卵巢癌组织的DNA指数 (DI)和DNA倍体组成进行测量和分析。结果卵巢癌细胞的DNA指数 (HI)在术前未化疗组和术前化疗组中分别为 3 6 5 4 1± 1 30 6 3,2 2 0 2 0± 0 6 0 38,两组比较差异有极显著意义 (P <0 0 0 1 ) ;DNA倍体构成在化疗前后差异也有显著意义 (P <0 0 0 5 ) ,前者峰值体现于 >5C的非整倍体 ,后者主要位于 3C～ 4C的整倍体群中。结论 (1 )反映肿瘤细胞增殖状态的DNA指数和DNA倍体组成可能与卵巢癌的发生发展之间有一定的相关关系 ,他们可能反映了肿瘤细胞对抗肿瘤药物的敏感程度和耐受程度 ,在卵巢癌的病理诊断 ,恶性程度判定 ,疗效评价及生物学行为和预后评估中可能有重要价值。 (2 )卵巢癌术前化疗再结合手术和观测癌细胞DNA含量的变化 ,可能为选择术后化疗方案提供依据 ,为提高卵巢癌患者的 5年生存率 ,成为将来临床上对卵巢癌可能有前景的治疗手段之一。
Objective To study the DNA contents in ovarian carcinoma and the relation to the chemotherapy and prognosis. Methods DNA index(DI) and DNA ploid was qualitatively analysed in 45 cases which 25 cases with chemotherapy and 20 cases without chemotherapy by means of the image analysis.Results DI were 3.6541±1.3063, and 2.20020±0.6038 in above mentioned ovarian carcinoma respectivly ( P <0.001). DNA ploid also have great changes between two groups ( P <0.005), DNA ploid in the former group without chemotherapy was mainly beyond 5C, but most of the latter was 3C-4C. Conclusion (1) DI and DNA ploid might be closely related to the occurrence and develotment of ovarian carcinoma. They might be reflect the sensitivity and resistance of tumor cells to the drugs. It may have great value in pathology-diagnosis, malignant judement, evaluation of curative effect, biology nature and prognosis. (2) The methods of chemotherapy associated of DNA contents may be the basement to chose the chemotherapy scheme after surgery.
出处
《贵州医药》
CAS
2004年第5期403-405,共3页
Guizhou Medical Journal
基金
贵州省科委科研经费资助 基金编号 ( 3 0 92 )
