心血管危险因子与广西汉族迟发性阿尔茨海默病之间的相关性研究

A Genetic Association Study between the Cardiovascular Risk Factor and Late-onset Alzheimer Disease in Guangxi Han Chinese

目的 研究心血管危险因子载脂蛋白 E( Apo E)、载脂蛋白 C- ( Apo C- )以及低密度脂蛋白受体相关蛋白 ( LRP)与汉族迟发性阿尔茨海默病 ( LOAD)之间的相关性。方法 应用 PCR-RFLP技术或直接通过 PCR方法 ,分析了 1 5 6例正常老年人、79例 LOAD Apo E、Apo C- 与 LRP基因型。结果 在 LOAD患者组和正常对照组中 ,Apo Eε4等位基因出现的频率分别为 1 7.7%、5 .7% ,其间差异有显著性 ( x2 =1 5 .75 0 ;P=0 .0 0 1 ,OR=3 .3 96,95 % CI =1 .80 8～ 6.3 79) ;Apo Eε3等位基因频率分别为 76.0 %、84% ,前者低于后者 ( x2 =4.43 9;P=0 .0 3 5 ,OR=1 .65 9,95 % CI =1 .0 3 3～ 2 .665 ) ;LRP、Apo C- 基因的基因型频率和等位基因频率在 LOAD与对照组之间差异无显著性 ( P >0 .0 5 )。结论 Apo Eε4等位基因是 LOAD的遗传危险因子 ,Apo Eε3等位基因则可能有保护效应 ;此研究未能证明 Apo C- 基因变异和 LRP基因 C766T多态性与

Objectives To explore the association of the cardiovascular risk factor (apolipoprotein E (ApoE), apolipoprotein C-Ⅰ(ApoC-Ⅰ) and low-density lipoprotein receptor-related protein (LRP) with late-onset Alzheimer disease (LOAD) in Han Chinese. Methods The genotypes of ApoE, ApoC-Ⅰ and LRP gene were analyzed in 79 late-onset Alzheimer disease patients and 156 healthy controls, using PCR and restriction fragment length polymorphism techniques. Results Among the LOAD group and the controls group, the fre-quency of ApoEε4 allele was 17.7%,and 5.7%,respectively.There was an obvious difference between them (x 2=15.750; P=0.001, OR=3.396, 95% CI =1.808-6.379);The frequency of ApoEε3 in the controls was higher than that in the LOAD group(84.0% vs 76.0%, P =0.035). However, the frequencies of LRP and ApoC-Ⅰ genotypes and alleles showed no significant difference between the two groups. Conclusions This study shows that ApoEε4 allele is one of genetic risk factor of LOAD and ApoEε3 may have protective effect on thedevelopmentofLOAD;thestudycannotclarify the relationship between the polymorphisms of ApoC-Ⅰ/ LRP gene and LOAD.