化学点燃癫痫大鼠在水迷宫中学习记忆能力与海马中bFGF表达的关系

The Examination of the Relation of Learning and Memory in Picrotoxin-kindled Rats after the Morris Water Maze Task Training to the Expression of bFGF in the Hippocampus

目的 观察印防己毒 ( picrotoxin,PTX)化学点燃癫痫大鼠在水迷宫中学习记忆训练与大鼠海马中碱性成纤维细胞生长因子 ( basic fibroblast grouth factor,b FGF)表达变化的关系 ,为进一步研究癫痫患者记忆损害及其治疗提供线索。方法 44只雄性 SD大鼠随机分为点燃组和对照组 ,分别用 PTX和生理盐水腹腔注射 ,根据点燃情况点燃组再分为全面发作 ( A)、频繁发作 ( B)及部分发作 ( C) 3组和迷宫对照 ( D)组 ,后者再分为迷宫训练 ( E)组和对照组 ( F)。然后进行水迷宫行为测试评价其学习记忆能力。用免疫组化和原位杂交法测定各组大鼠海马中 b FGF表达变化。结果 癫痫大鼠在水迷宫测定中 ,除 B组第 1天的成绩较对照组差外 ,其余各组及 B组在第 2、3、4、5天中寻找平台的潜伏期时间与对照组相比差异无显著性。点燃各组对平台空间位置记忆的能力较对照组要差 ,差异有显著性。大鼠海马中 ,b FGF免疫组化染色阳性细胞在点燃各组和 E组表达增高 ,b FGFm RNA在点燃各组和 E组表达增强 ,而以 A、B、C组表达增高最明显。结论 首次用化学点燃模型研究癫痫大鼠在水迷宫中的学习记忆能力 ,发现 PTX化学点燃癫痫大鼠在水迷宫中学习记忆能力下降 ,发作频繁者学习记忆受损明显 ,与发作的严重程度无关。水迷宫训练可使?

Objective To observe the relation of the learning and memory in picrotoxin-kindled rats during the Morris water maze task training with the expression of basic fibroblast grouth factor(bFGF) in hippocampus. Methods 44 male SD rats were randomly divided into kindle group and control group. Picrotoxin were injected intraperitoneally for kindle groups,which according to the state of the seizure, were divided into group A (general seizure group), group B (frequent seizure group) and group C (partial seizure group), group D (seizure, maze-control group), group E (maze group), and group F was salinecontrol group). Then the learning and memory of group A, B, C and E were tested after Morris water maze training. Then the learning and memoryabilitiesweretestedafterMorriswatermazetraining.TheexpressionlevelsofbFGFinthehip-pocampus of the rats of different group were compared by use of in situ hybridization and immunohistochemical techniques. Results during the first day of place navigation, rats in group B performed worse than the control group ( P <0.05), otherwise, there was no difference between all kindle groups (including group B during the other 4 days) and control group in the latency of escape. In the spatial probe test, the times across the original platform of the kindled rats were fewer than control group. The expression level of bFGF protein and mRNA in hippocampus of rats was increased in group ABCDE as compared with control group. Conclusions The ability of the learning and memory in picrotoxin-kindled rats assessed after the Morris water maze task training in our hands were shown impairing. They were associated with seizure frequency, but in no relation with seizure type. Water maze training might increase the expression level of bFGF protein and bFGF mRNA. It might play a protect role in the memory impairment.