摘要
目的 探讨癫痫大鼠海马中神经肽 Y( NPY)表达的动态变化及其作用 ,以及外源性 NPY对癫痫的影响。方法 将 SD大鼠随机分为 4组 ,采用匹鲁卡品 ( PILO)癫痫动物模型及外源性 NPY侧脑室注入干预法 ,并以免疫组织化学染色观察 NPY的表达。结果 癫痫模型组 PILO注射后 ,门区、CA3区、CA1 区的 NPY阳性细胞数表达增多 ,1 2 h达高峰 ,以后逐渐下降 ,60 d又达一高峰 ;海马颗粒细胞层有 NPY异位表达 ,3 d时最明显 ;NPY干预组门区 NPY阳性细胞数较其对照组表达少。结论 ( 1 )癫痫发作后急性期海马 NPY表达增多及颗粒细胞层出现异位表达 ,与癫痫发作有关 ;( 2 )外源性 NPY有抗癫痫作用。
Objective To explore the dynamic changes of NPY expression and its action against the seizures and the influence of extrogenous NPY to the epileptic seizures. Methods Epileptic animal models of adult Sprague-Dawley rats were induced by intraperitoneal injection of pilocarpine, which were divided into four groups including extrogenous NPY interference group. The expression of NPY was investigated by immunohistochemical staining. Results NPY positive cells were increase in the hilus, CA 1 and CA 3 subfield, which expressed dynamicly and aberrant expression of NPY was seen in the granular cell layer of hippocampus in epilepsy group after PILO injection. The number of NPY positive cells in the NPY interference group was fewer than its control group. Conclusions (1)The expression of NPY positive cells was increased after seizures, and aberrant expression of NPY was seen in the granular cell layer of hippocampus, which seemed to be related to seizures.(2)The extrogenous NPY could inhibit the occurrence of the seizures.
出处
《中国神经免疫学和神经病学杂志》
CAS
2004年第2期76-79,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
湖南省科学技术委员会课题基金资助项目 ( 99SSY10 10 )
教育部高校骨干教师基金资助项目 ( 2 0 0 0
No.65 )
