1,25-二羟维生素D_3对骨髓破骨细胞形成和破骨细胞分化因子mRNA表达的影响

Effects of 1,25-(OH)_2D_3 on stimulation of osteoclast formation and expression of ODF in murine marrow cell

目的 :观察不同浓度的 1,2 5 - (OH) 2 D3 对骨髓单个核细胞诱导形成破骨细胞和对单个核细胞ODFmRNA表达的影响。进一步阐明骨吸收刺激因子在破骨细胞性骨吸收中的作用机制。方法 :应用不同浓度的 1,2 5 - (OH) 2 D3 ( 0、10 -10 、10 -8、10 -6mol/L)诱导大鼠破骨细胞的形成 ,观察形成破骨细胞的数目 ,并采用原位杂交技术检测骨髓细胞ODFmRNA的表达。结果 :随着 1,2 5 - (OH) 2 D3 浓度的增加 ,多核细胞计数增多 ;ODFmRNA表达的阳性信号显著增强。结论 :1,2 5 - (OH) 2 D3 通过调节骨髓细胞ODFmRNA的表达 ,影响破骨细胞的形成和功能 ,进而调节局部骨微环境内骨吸收与骨形成平衡的变化 ,影响骨组织改建。

AIM:To investigate the effects of 1,25-(OH) 2D 3 on stimulation of osteoclast cell formation and expression of ODF in murine marrow cell,and further clarify the mechanisms of osteoclastic bone resorption regulated by 1,25-(OH) 2D 3.METHODS:Investigate the influence of different concentrations of 1,25-(OH) 2D 3 (010 -10 ?10 -8 ?10 -6 mol/L)on the number of oteoclast formation.The effects of 1,25-(OH) 2D 3 on the expression of ODF in murine marrow cell was examined by in situ hybridzation technique.RESULTS:1,25-(OH) 2D 3 could induce recruitment of osteoclast cell.The number of oteoclast formation increased and the expression of ODF mRNA increased with increasing concentration of 1,25-(OH) 2D 3 (0?10 -10 ?10 -8 ?10 -6 mol/L).CONCLUSION:1,25-(OH) 2D 3 could modulate the recruitment of osteoclast and the expression of ODF mRNA in murine marrow cell,thus adjust the bone metabolism.