茉莉酸甲酯对烟草幼苗抗病毒的影响

Effect of Methyl Jasmonate on the Resistance of Tobacco Seedlings to TMV

用茉莉酸甲酯处理4叶期烟草(NicotianatabacumL.)幼苗后接种烟草花叶病毒,考察发病情况和病情指数,并测定一些与抗病相关的酶活性。结果表明,茉莉酸甲酯处理后接种病毒明显降低巴西烟草的病情指数,提高几丁内切酶、β1,3-葡聚糖苷酶、SOD、脂氧酶的活性。其中仅SOD活性与抗病毒有较密切的关系。茉莉酸甲酯可能是诱导巴西烟草抗花叶病毒的信号物质。

Four-leaf seedlings of tobacco were used to study the resistance to tobacco mosaic virus (TMV). After the seedlings were sprayed with 1 mg ml-1 methyl jasmonate (MJ) once a day for 3 days, then TMV was inoculated to the leaves. Among three tobacco varieties, brazil tobacco (BX) showed lower disease index than the other two (GH55 and K326). Endochitinase, β1,3-Glucanase, superoxide dismutase (SOD) and lipoxigenase activities increased in MJ-treated BX tobacco seedlings. It is suggested that MJ plays an important role in the increase of SOD and in the resistance of BX tobacco against TMV.