茶多酚、茶色素对人肝癌细胞株HepG2端粒酶活性的影响

Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells

目的 利用人肝癌细胞株HepG2观察茶多酚和茶色素对端粒酶活性的影响。方法采用TRAP PCR ELISA方法对HepG2细胞端粒酶活性进行定性和定量检测。结果 TRAP PCR定性分析表明空白对照组、茶多酚组和茶色素组均端粒酶阳性 ;ELISA定量结果显示 ,5 0mg/L和 10 0mg/L茶多酚组端粒酶活性 (A450 690 值 )分别为 1 5 6和 1 4 6 ;5 0mg/L和 10 0mg/L茶色素处理组为 1 5 5和1 4 9,与空白对照组 (A450 690 值为 2 11)相比 ,茶多酚和茶色素处理后HepG2细胞端粒酶活性有统计学意义。结论 茶多酚和茶色素显著抑制HepG2细胞端粒酶活性 。

Objective This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells Methods TRAP PCR ELISA was applied to investigate the telomerase activity Results Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group Telomerase activities ( A 450~690 values) were 1 56 and 1 46 in 50 mg/L and 100 mg/L tea polyphenols treated groups, 1 55 and 1 49 in 50 mg/L and 100 mg/L tea pigments treated groups, respectively The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group ( A 450~690 =2 11) Conclusions Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention