稳恒磁场对大鼠胚胎中脑神经细胞发育的影响

Effect of static magnetic field on development toxicity of rat embryonic midbrain neurons cells

目的 研究稳恒磁场对中脑神经细胞分化和增殖的磁场强度效应关系 ,并探讨其机制。方法 利用大鼠胚胎中脑神经细胞进行原代微团培养 ,观察不同磁场强度 1 0、10 0、5 0 0、10 0 0、2 0 0 0mT的稳恒磁场对细胞分化和增殖的影响 ,并利用图像分析细胞形态变化、硫代巴比妥酸测定丙二醛和流式细胞仪测定蛋白总量。结果 磁场强度 (≥ 10 0mT)会抑制胚胎中脑细胞分化和增殖 ,集落形成率明显减少、细胞体积小 ,并显示磁场强度效应关系 ,拟合细胞分化和增殖抑制曲线 ,计算得中脑神经细胞半数分化抑制磁场强度 (ICD50 )为 2 5mT ,半数存活抑制磁场强度 (IVD50 )为 4 5mT。

Objective To explore the effect of static magnetic field (SMF) on the differentiation and proliferation of rat embryonic midbrain neurons cells Methods The micromass culture of rat embryonic midbrain neurons cell was applied to study the effect of varieties of SMF(1 0,10 0,50 0,100 0,200 0mT) and FACS Results SMF inhibited the differentiation of the cell without affecting cell proliferation The concentration of 50% inhibition of cell differentiation (ICD 50 ) was 25 mT The concentration of 50% inhibition of cell differentiation (IVD 50 ) was 45 mT Conclusions The inhibition of SMF on embryonic midbrain neurons cells may be associated with impact of protein syntheses, and lipid peroxidation