增生性瘢痕转化生长因子β及其受体的分布及表达

Distribution and expression of transforming growth factor beta and their receptors in hypertrophic scar

目的 探讨转化生长因子 β(TGF β)及其受体在增生性瘢痕形成中的作用机制。 方法 收集临床手术切除后的正常皮肤组织 7例和增生性瘢痕组织标本 11例 ,用免疫组织化学和原位杂交的方法检测TGF β及其TGF β受体 (TGFR)Ⅰ、TGFRⅡ在组织中的定位分布、蛋白和mRNA表达。 结果 在正常皮肤组织中 ,与TGF β1 、TGF β2 、TGFRⅠ比较 ,TGF β3和TGFRⅡ呈较高表达 ;在增生性瘢痕组织中则呈现与正常皮肤组织中的表达相反的结果。与正常皮肤组织相比 ,增生性瘢痕组织中TGF β1 、TGF β2 和TGFRⅠ的蛋白和mRNA表达均明显增加 ,而TGF β3和TGFRⅡ的蛋白以及mRNA表达相对减少。 结论 创面愈合过程中TGF β及其受体不同水平的表达与增生性瘢痕的形成直接相关。

Objective To explore the role of transforming growth factor beta (TGFβ) and their receptors (TGFβ-R) in the pathogenesis of hypertrophic scar. Methods Specimens of normal skin and hypertrophic scar were harvested and the distribution and the expression of the TGFβ and TGFβ-R were determined by immunohistochemistry and in situ hybridization method. Results The expressions of TGFβ and TGFβ-RⅡ in normal skin were higher than the expression of TGFβ1, TGFβ2 and TGF-RⅠ. But in hypertrophic scar the results were on the contrary. The mRNA expressions of TGFβ1, TGFβ2 and TGFRⅠ were evidently increased with decreased mRNA expression of TGF-β3 and TGFR Ⅱ in the hypertrophic scar when compared with those in the normal skin. Conclusion The expression of TGF-β (β1, β2, β3) and their receptors in different levels during the process of wound healing might be related to the formation of hypertrophic scars.