茶皂素对壬苯醇醚杀精增效作用的实验研究

Experimental Research on Tea Saponin Ameliorating Nonoxynol Spermicidal Action in Vitro

目的 :观察茶皂素对壬苯醇醚 (N 9)的杀精增效作用。 方法 :按照国际计划生育基金会体外杀精试验改良法 ,测定N 9、茶皂素及其混合液对家兔精子 2 0s、3min的最低杀精浓度。 结果 :混合液中N 9、茶皂素于 2 0s、3min时的最低杀精浓度分别为 (0 .13± 0 .0 5 )、(2 .4 0± 1.0 7)g/L和 (0 .0 5± 0 .0 1)、(1.2 7± 0 .38)g/L ,均明显低于各自于 2 0s、3min时的单独使用剂量 (0 .4 8± 0 .15 )、(5 .78± 1.4 0 )g/L和 (0 .34± 0 .0 8)、(1.71± 0 .18)g/L ,P均 <0 .0 1。 结论 :茶皂素对N 9体外杀精避孕具有增效作用 ,两者有明显的协同作用。

Objective: To study the effect of tea saponin in ameliorating nonoxynol(N-9) spermicidal action in vitro. Methods: According to the improved spermicidal test method in vitro recommended by International Planned Parenthood Foundation (IPPF), we evaluated the minimum spermicidal concentration of N-9, tea saponin and their mixed solution in 20 s and 3 min. Results: The minimum spermicidal concentration of N-9 in the mixed solution was ( 0.13± 0.05) g/L in 20 s and ( 0.05± 0.004) g/L in 3 min, and that of the tea saponin in the mixed solution was ( 2.40± 1.07) g/L in 20 s and ( 1.27± 0.38) g/L in 3 min,compared with the single ingredient N-9 [( 0.48± 0.15) g/L in 20 s, ( 0.34± 0.079) g/L in 3 min], and tea saponin [( 5.78± 1.40) g/L in 20 s, ( 1.71± 0.176) g/L in 3 min], P< 0.01. Conclusion: Tea saponin can improve N-9 spermicidal action in vitro, and tea saponin and nonoxynol have proved of synergic effect.