同种异体双手移植术细胞因子的动态监测

DYNAMIC MONITORING OF sIL-2R, IL-2 AND IL-6 LEVELS OF SERUM AFTER DOUBLE-HAND TRANSPLANTATION

目的 探讨同种异体双手移植术细胞因子动态监测的临床应用价值。 方法 2 0 0 0年 9月～ 11月 ,采用 EL ISA法动态监测异体手移植术患者移植前后 ,血清中可溶性白细胞介素 - 2受体 (solube interleukin- 2 ,receptor,s IL- 2 R)、白细胞介素 - 2 (interlukin- 2 ,IL- 2 )和白细胞介素 - 6 (IL- 6 )水平的变化 ,并与 15名健康对照血清中相应细胞因子水平进行比较。 结果 血清 IL- 2和 IL- 6水平于术后诱导期明显下降 ,与术前比较有统计学意义 (P<0 .0 5 ) ,而后回升 ,在维持期保持较术前略高水平 ,与诱导期比较有统计学意义 (P<0 .0 5 ) ;s IL- 2 R水平术后明显上升 ,并维持于较高水平。血清 IL- 2水平与对照组比较 ,在移植前后均有统计学意义 (P<0 .0 1) ;IL- 6水平与对照组比较 ,术后诱导期有统计学意义 (P<0 .0 5 )。 结论 血清 IL- 2、IL- 6和 s IL- 2 R的动态监测可能有助于排斥反应的诊断 ,以及预防机会性感染、肿瘤及移植物抗宿主疾病的发生。

Objective To investigate the value of monitoring cytokines in serum after human double-hand allotransplantation. Methods We detected solube interleukin-2 receptor(sIL-2R), interleukin-2(IL-2) and interleukin-6(IL-6) levels of the serum in the patient who were performed double-hand transplantation with immunosupressive from September 2000 to November 2000, and in 15 healthy volunteers by using enzyme linked immunosorbent assays. Results The IL-2 and IL-6 levels decreased quickly during inducing period (P<0.05), and then increased gradually. While the sIL-2R level increased. The IL-2 level was lower(P<0.05), the IL-6 and sIL-2R levels were slightly higher when compared with those before transplantation. There was significant difference in the IL-2 level between the patient and healthy volunteers both before and after transplantation, in the IL-6 level between the patient and healthy volunteers during inducing period. Conclusion IL-2, IL-6 and sIL-2R levels of serum may be useful in diagnosis of rejection, prevention of opportunistic infection, tumor and graft versus host disease.