硬组织切片技术在组织工程骨研究中的应用

APPLICATION OF HARD TISSUE SLICING IN RESEARCH OF BONE TISSUE ENGINEERING

目的 改进硬组织切片技术以适应骨组织工程研究。 方法 组织工程骨修复兔桡骨的术后标本和四环素标记大鼠股骨标本的观察。通过对硬组织切片中组织包埋、切片的方法、步骤及染色法 ,确定操作过程中的关键技术和需注意的问题 ,重点解决硬组织切片易于脱片的缺点。 结果 经改进后的硬组织切片技术能保持其原有组织结构 ,可进行 HE、Masson法以及免疫组织化学染色 ,染色后组织结构完整 ,细胞形态清晰 ,切片质量好。荧光显微镜观察骨组织结构完整。克服了普通硬组织切片技术容易发生脱片的缺点。 结论 改进的硬组织切片技术适合骨组织工程研究。

Objective To improve the method of hard tissue slicing for research of bone tissue engineering.Methods The method of hard tissue slicing especially the key technique-slice mounting had been studied and improved. Results The experiments showed that our research improved techniques in hard tissue slicing not only in keeping the characteristics of hard tissue slicing, easily stain with HE, Masson, immunohistochemistry, and distinguishing the differences among tissues easily, but also in overcoming the shortcoming of issue shedding easy to happen in the general hard tissue slicing. Conclusion The improved techniques in hard tissue slicing is a more useful hard tissue slicing techniques.