白细胞介素4及肿瘤坏死因子-α基因多态性与湖北汉族儿童哮喘相关性研究

Correlation between Polymorphisms in the IL-4 Promoter Region and Tumor Necrosis Factor alpha Gene and Susceptibility of Allergic Asthma in Chinese Children of Han Nationality in Hubei Province

目的 探讨白细胞介素 (IL) 4基因启动子区C +33T及肿瘤坏死因子 α (TNF α) 30 8位基因多态性与湖北汉族儿童哮喘的易感性的关系。方法 用聚合酶链反应和限制片段长度多态性 (PCR/RFLP)的方法对湖北汉族115例哮喘患儿及 75例健康儿童进行IL 4启动子的 +33位点多态性分析 ,对 113例哮喘患儿及 12 6例健康儿童进行TNF α的 30 8位点多态性分析。结果 哮喘患儿与健康对照组IL 4基因启动子C +33T位点的基因型频率和等位基因频率CC、CT、TT之间有显著性差异 (P <0 0 0 1)。TNF α的 30 8位点的基因型频率和等位基因频率在两组的比较中无显著性差异 (P >0 0 5 )。结论 IL 4启动子 +33位点基因多态性与湖北汉族儿童哮喘易感性相关 ,TNF α 30

Objective To investigate the correlation between the polymorphism of IL 4 promoter region C+33T and tumor necrosis factor α (TNF α) 308 (G→A) gene polymorphism and the susceptibility of Chinese children with asthma. Methods The polymorphisms in the IL 4 promoter region and TNF α were determined by PCR/RFLP in Han nationality of Hubei Chinese population. 115 asthmatic children and 75 age-matched healthy children without asthma as controls were subjected to IL 4 gene analysis, and 113 asthmatic children and 126 age matched healthy children to TNF α gene analysis. Results There was significant difference in the allele frequency of +33 (C/T) site of IL-4 gene promoter region and genotype frequency CC, CT, TT between asthma children and controls ( P <0 001). However, there was no significant difference in the allele frequency and genotype of TNF α 308 ( P >0 05). Conclusion The C+33T polymorphism in the IL 4 promoter region is associated with susceptibility of allergic asthma, but TNF α 308 gene polymorphism not with susceptibility of allergic asthma in Chinese children of Han nationality in Hubei province.