不同时期切痂对烫伤大鼠骨骼肌和脂肪组织葡萄糖转运蛋白-4mRNA表达水平的影响

Effects of the escharectomy during burn shock stage on expression of glucose translator-4 mRNA in skeletal muscle and adipose tissue

目的 探讨大面积烫伤后实施休克期切痂对大鼠骨骼肌、脂肪组织葡萄糖转运蛋白 4(GLUT4 )mRNA表达水平的影响。方法 采用 30 %Ⅲ度TBSA烫伤大鼠模型 ,分别于伤后 8、2 4、96h切痂 ,测定血清胰岛素、胰高血糖素、皮质醇及血糖水平 ,采用RT PCR法检测烫伤和切痂后大鼠骨骼肌、脂肪组织GLUT4mRNA表达水平。结果 休克期切痂使血糖、胰高血糖素、皮质醇水平下降 ;伤后骨骼肌、脂肪组织GLUT4mRNA表达水平下降 ,休克期切痂可促使其回升。结论 大面积烫伤后大鼠骨骼肌、脂肪组织GLUT4mRNA表达减少 ,休克期切痂可促使其恢复 ,有利于糖代谢的改善 ,减轻高代谢状态。

Objective To investigate the effect of the escharectomy during burn shock stage on expression of glucose translator-4 (GLUT4) mRNA in skeletal muscle and adipose tissue. Methods 30% TBSA scalded rats were employed. Escharectomy were conducted at 8 h,24 h, 168 h after burns respectively. Insulin, glucagon, cortisol and glucose levels in serum were analyzed. RT-PCR were employed to analyze GLUT4 mRNA expression in skeletal muscle and adipose tissue. Results Glucagon, cortisol and glucose levels in serum were declined in groups which escharectomy were conducted during burn shock stage. GLUT4 mRNA expression in both skeletal muscle and adipose tissue were downregulated after burns and escharectomy conducted during burn shock stage made it restored to near normal. Conclusion GLUT4 mRNA expression will declined after major burns in skeletal muscle and adipose tissue. Escharectomy during shock stage could make it upregulated, which will be helpful to improve glucose metabolism and hypermetabolism after major burns.