选择性深低温断血流对猴神经功能及神经元超微结构的影响

Effects on neuronal ultrastructure and nervous system of monkey after selective cerebral profound hypothermia and blood flow occlusion

目的 研究选择性深低温断血流对猴神经功能及神经元超微结构的影响。方法 建立闭胸式脑局部体外循环 ,一侧颈内动脉冷灌注 ,同侧颈内静脉回流 ,6 0～ 80min后恢复脑血流 ,实验动物自然复苏。手术前后监测各主要脏器的血生化变化、观察神经功能并在预定的时间内处死动物行全脑电镜检查。结果 7只猴中 2只未能建立实验模型 ,其余 5只均于术后复苏 ,其中 4只长期存活 ;各主要脏器功能未见显著变化 ,术后神经功能评分无异常 ,神经元超微结构无明显异常。结论 脑选择性深低温可明显提高脑对缺血缺氧的耐受性 ,延长血流阻断的时间。

Objective To study the effects of deep hypothermia on the neuronal ultrastructure and nervous system of monkey after selective cerebral profound hypothermia and blood flow occlusion. Methods Brain-local extracorporeal circulation was established by right internal carotid artery deep hypothermic perfusion and homolateral external jugular vein backflow,brain blood flow was recovered from circulatory arrest 60～80 minutes late and monkey came back naturally. Results In all 7 monkeys,5 were succeeded in being build up the models except for 2 because of technic problems,and 4 of them lived up for ever.The function of nervous system grade,essential organ and neuronal ultrastructure were normal. Conclusion Selective cerebral profound hypothermia can increase the ability of brain to endure hypovolemia and hypoxidosis and prolong the time of blood flow occlusion.