皮肤血管瘤内皮细胞增殖和凋亡的研究

A study of proliferative and apoptoticStatus in hemangioma of skin

目的 探讨细胞增殖和凋亡状态与皮肤血管瘤发生和发展的机制。方法 采用免疫组织化学方法和末端脱氧核苷酸转移酶介导的末端标记技术 (TUNEL) ,检测 40例不同时期婴幼儿皮肤血管瘤及 2 0例周围正常皮肤组织内皮细胞增殖和凋亡状态。结果 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及 2 0例正常皮肤组织平均增殖指数分别为 60 .5 8、1.47及 1.49,经配对t检验差异具有高度显著性 (P <0 .0 0 1)。 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及2 0例正常皮肤组织平均平均凋亡指数分别为 0 .0 97、1.0 2 1及 1.0 46,经配对t检验差别具有高度显著性 (P <0 .0 0 1)。结论 皮肤血管瘤的血管内皮细胞异常增生和凋亡在血管瘤的发生、发展和退化过程中起着重要作用 ,为临床治疗血管瘤提供新的思路。

Objective To explore the mechanism of formation and development of hemangioma,proliferating cell nuclear antigen (PCNA)and cell necrosis were studied in this paper.Methods The proliferation of endothial cell and necrosis condition of hemangioma were detected with immunohistochemistry method and TdT-Mediated dUTP Nick End Labeling (TUNEL) in 40 infants and children of different stage and the PCNA positive and necrosis cells were counted.Results The results indicated that there were 20 samples in proliferative phase and 20 samples in degenerative phase of all 40 hemangioma samples.The mean proliferative index number of 20 hemangioma of proliferative phase is 60.58 and those of 20 hemangioma in degenerative phase and 20 normal skin tissues are 1.47 and 1.49.The difference of mean proliferative index number between hemangioma groups and normal skin group is significant (P<0.001).Conculion The abnormal proliferation and necrosis of endothelial cells in hemangioma played an important role in formation and development of hemangioma.This provided important theory bases for further study of pathology and treatment of hemangioma.