癌基因产物c-jun和c-fos在髓母细胞瘤中的表达及临床意义

Expression of c-jun and c-fos oncogene in medulloblastoma and its clinical significance

目的 了解c jun和c fos在髓母细胞瘤中的表达情况及其与预后的关系。方法 通过免疫组化方法测定c jun和c fos蛋白在 70例髓母细胞瘤和 10例正常小脑组织中的表达情况 ,并结合临床资料 ,对病人生存时间与c jun和c fos表达阳性率之间的关系进行分析。 结果 (1)正常小脑组织标本不表达c jun和c fos;而髓母细胞瘤中多为不同程度的阳性表达 ,且以高阳性率为主 ,并发现阳性染色主要位于肿瘤细胞核 ,部分胞浆也有染色。 (2 )c jun和c fos表达呈正相关 (r =0 4 93,P <0 0 1) ;二者协同性较强。 (3)生存时间与c jun、c fos表达阳性率呈负相关 (c jun :r =- 0 4 4 7,P <0 0 1;c fos:r =- 0 5 90 ,P <0 0 1) ;c jun、c fos表达强度愈高 ,病人的生存率就愈低 ,生存时间就越短。结论 c jun和c fos在髓母细胞瘤组织中呈高表达。c jun和c fos二者在髓母细胞瘤中表达具有协同性。c jun、c

Objective To study the potential relationship between the expressions of c jun and c fos oncogenes and the prognosis of medulloblastoma Methods The specimens from 70 cases of medulloblastoma of the posterior fossa and 10 cases of normal cerebellar tissues were collected to determine c jun and c fos expressions by immunohistochemical staining in formalin fixed paraffin embeded sections Results (1) It showed that c fos and c jun protein expression was negative in 10 normal cerebellar tissue, while positive c fos, c jun immunoreactivity was found in 70 medulloblastoma specimens The positive rate of c jun and c fos was 80% and 77%, respectively There was high expression of c jun and c fos protein in medulloblastoma tissues (2) There were positive correlations and strong co operativity between c jun and c fos expression ( r =0 493, P <0 01) (3) Correlative analysis indicated that expression of c jun, c fos were significantly correlated with survival time (c jun: r =-0 447, P <0 01; c fos: r =-0 590, P <0 01) The higher the expression level of c jun and c fos protein was, the worse the prognosis was in medulloblastoma patients Conclusions High expression of c jun and c fos protein could be noted in medulloblastoma tissues The two transcription factors show positive correlation and strong co existence between c jun and c fos expressions The expression levels of c jun as well as c fos are negatively correlated with the mortality rate and life expectancy of patients with medulloblastoma In addition, the co expression of c jun and c fos could serve as an indicator for judging the prognosis of medulloblastoma