构树叶总黄酮对表皮细胞防护作用研究

Study on Protective Effects of TFBP on Human Immortalized Keratinocytes Line HaCaT

目的研究构树叶提取物构树总黄酮(totalflzvonoidsofbroussonetiapapyrifera,TFBP)对铅、砷染毒的人永生化表皮细胞氧化损伤的防护效果。方法在人永生化表皮细胞培养的基础上,在0.1mmol/L的醋酸铅、5.0μmol/L的亚砷酸钠的染毒体系中,分别加入0～200mg/LTFBP,测定细胞裂解液中丙二醛(MDA)含量和超氧化物歧化酶(SOD)和谷胱甘肽过氧化酶(GSH鄄Px)的活力,评价TFBP的抗氧化效果。结果0.1mmol/L的醋酸铅对细胞染毒产生氧化损伤,而添加高于100mg/LTFBP后,MDA含量由4.23nmol/mgPro降低到1.87nmol/mgPro,SOD活力由25.90U/mgPro增加到37.12U/mgPro,但GSH鄄Px的活力变化不大。砷+TFBP150mg/L、200mg/L组与砷染毒组相比,GSH鄄Px和SOD活力差异有显著性。结论该试验条件下,TFBP对铅、砷染毒的人永生化表皮细胞氧化损伤有防护效果。

Objective To investigate the oxidative damage of lead acetate and sodium arsenite to human immortalized keratinocytes line HaCaT and the protective effects of TFBP extracted from the leaves of broussoneria papyifera. Methods Cultured immotlalized keratinocytes line HaCaT were treated by 0.1 mmol/L lead acetate and 5.0 μmol/L sodium arsenite respectively,and 0～200 mg/L TFBP were added to the culture media at the same time. The contents of malondialdehyde(MDA?雪,the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the cultured HaCaT cells were determined. The protective effects of TFBP at different concentrations were evaluated. Results 0.1 mmol/L lead acetate caused oxidative damage to HaCaT cells markedly. When the concentrations were increased to more than 100 mg/L,TFBP had certain protective effects from the damage induced by lead acetate with the decrease of the MDA levels from 4.23 μmol/L to 1.87 μmol/L and the increase of the SOD levels from 25.90 U/mg Pro to 37.12 U/mg Pro,while the activities of GSH-Px showed no significant change. The activities of SOD and GSH-Px were increased in the cultured cells treated by sodium arsenite with 150 mg/L and 200 mg/L TFBP. Conclusion Lead acetate and sodium arsenite could cause significant oxidative damage to HaCaT cells and TFBP had certain protective effects on the cells from the oxidative damage induced by lead acetate and sodium arsenite under the conditions of this study.