二噁英类污染物的CALUX测定法

An Easy Method for Determinaion of Dioxins:the CALUX Assay

该文介绍了一种利用转基因细胞的生物学特性测定样品中二叶恶英总毒性当量的生物学方法———CALUXTM法。资料表明CALUX方法和国际上公认的二叶恶英类标准测定方法———高分辨率气相色谱/质谱(HRGC鄄MS)法测定同一样品二叶恶英含量的结果之间具有很好的正相关,且具有很好的重现性。与HRGC鄄MS方法相比,CALUX法具有所需样品量较小,测定周期短,测定成本低等优点。CALUX法适用于对大批量样品二叶恶英含量的快速定量筛选,对于环境中二叶恶英污染调查有广阔的应用前景。

This article introduces a new method: the chemical-activated luciferase gene expression (CALUX)assay for determine of dioxin,which is based on biological properly of a genetically modified cell line.The published thesis reports that the contents of dioxins in a same sample abtained from the CALUX assay and the internationally recoganized standard method for determination of dioxin: high resolution gas chromatography-mass spectrometry(HRGC-MS)showed perfectly positive correlation and well repeatibility. The CALUX assay presents less amount-required sample,shorter determination cycle and less expense compared with HRGC-MS assay. The CALUX assay can be used as a rapid screen assay for determination of dioxin in a large number of samples,and will be widely applied in the survey of dioxin pollution in environment.