家兔回肠淋巴管铸型的扫描电镜研究

SCANNING ELECTRONMICROSCOPIC STUDIES ON LYMPHATIC CORROSION CASTS IN RABBIT ILEUM

目的 :观察家兔回肠淋巴管的三维结构和微细分布。方法 :淋巴管铸型样品 ,通过扫描电子显微镜进行观察 ;半薄切片样品 ,用光镜进行观察。淋巴管铸型剂是Mercox ,采用回肠壁间接注射法 ,将注入铸型剂的回肠壁切下 ,置入NaOH水溶液中腐蚀 ,直到肠壁组织完全脱离为止 ,然后将淋巴管铸型样品置于扫描电镜下观察。结果 :在小肠绒毛内清晰地显示出中央乳糜管 ,每个绒毛中有 2～ 3个中央乳糜管 ,中央乳糜管与粘膜层毛细淋巴管丛相连通 ,粘膜层毛细淋巴管注入粘膜下层淋巴管 ,后者与肌层淋巴管相吻合 ,肌层淋巴管连接浆膜层淋巴管 ,然后汇入小肠系膜淋巴管。淋巴管呈串珠样外观 ,其表面存在双凹切迹 ,该处相当于淋巴瓣的部位。铸型表面还可以见到淋巴管内皮细胞核的压迹。结论 :淋巴管铸型清晰地显示家兔回肠壁粘膜层丰富的中央乳糜管和毛细淋巴管丛以及粘膜下层。

Objective: To investigate the three-dimensional organization and fine Distribution of the lymphatics in rabbit ileum. Methods: Lymphatic corrosion cast with the Mercox were used for scanning electron microscopy (SEM), and semithin sections were used for light microscopy . The Mercox injected intraparenchymally into ileum wall were cut and put in a concentrated NaOH solution until the tissues were corroded away, and observed under SEM. Results: The central lacteals were found in the intestinal villi. The villi of the ileum contained two to three lacteals. The central lacteals were drained into the mucosal lymphatic capillary plexus. From the plexus, the lymphatic capillary descended into the lymphatics of submucosal layer and muscular layer. Then they were led into the serosal lymphatics and drained into the lymphatics of intestine mesentery. The cast of the lymphatics showed an appearance of a string of heads and the notch corresponding to the bicuspid valve of the lymphatics. Conclusion: The three-dimensional organization of central lacteals and lymphatics in the rabbit ileum is demonstrated by lymphatics corrosion casts.Numerous impressions of the endothelial nuclei, rich central lacteals and lymphatics capillary plexus in the ileum mucosa, lymphatic capillary and lymphatics in the submucosa and muscular layer are observed on the lymphatic corrosion cast.