致癌基因与乌骨鸡“乌色”性状的相关

Association of Oncogene with "Black"haracter in Chicken

为探寻与乌骨鸡“乌色”性状相关的基因,从基因差异表达入手,比较全同胞乌骨鸡与非乌骨鸡的基因表达情况,通过对差异片段的回收、再扩增、纯化以及相应的克隆、测序与序列分析,获得2个表达序列标签(EST),它们分别与鸡插入激活c-Ha-ras致癌基因和fra-2致癌基因同源,同源性均为98%.根据测序结果设计特异性引物,利用12个随机采集的样本,进行基因表达分析.结果表明,插入激活c-Ha-ras致癌基因具有非乌骨鸡表达特异性,fra-2致癌基因没有乌骨鸡或非乌骨鸡表达特异性.

To isolate genes related to 揵lack攃haracter in chicken,the study compare gene expression between black chicken and non-black chicken and obtain 2 ESTs by cloning and sequencing.The 2 ESTs have hign homology with chicken insertionally activated c-Ha-ras oncogene and fra-2 oncogene respectively with 98% homology rate.The results of gene expression show that c-Ha-ras oncogene is especially expressed in non-black chicken and fra-2 oncogene is not especially expressed in non-black chicken and black chicken.