福建省2株麻疹病毒的分离与鉴定

Isolation and Identification of 2 Strains Measles Viruses in Fujian

[目的 ]了解福建省目前流行的麻疹野病毒的型别与抗原性。[方法 ]细胞培养分离病毒 ;PT PCR扩增病毒N基因碳末端 45 0个核苷酸 ,并对其产物测定基因序列以定型 ;细胞中和试验进行抗原性分析。[结果 ]B95a细胞分离到了 2株麻疹野病毒 ,Vero细胞未分离到 ,表明B95a细胞敏感性较高 ;分离到的病毒经PT PCR及基因序列测定证实为麻疹野病毒H1基因型 (中国流行的优势株 ) ;分离的野病毒株与疫苗株同时进行血清中和试验显示 :目前使用的疫苗产生的抗体对流行的野病毒仍具有中和作用 ,但中和滴度低于疫苗株。[结论 ]在保证生物安全的前提下 ,B95a细胞是目前分离麻疹病毒的首选细胞 ;新变异来的麻疹H1基因型也在我省流行 ,且目前使用的疫苗所产生的抗体 ,在体外中和野毒株的滴度低于疫苗株。

Objective\] To explore the genotype and antigenicity of epidemic measles viruses in Fujian \ Viruses from urine specimens of measles patients were isolated using B95a and Vero cell lines 450 nucleotides of COOH terminal of nucleoprotein genes were amplified and sequence analyzed by RT PCR and sequence analysis software And virus antigenicity was determined by serum neutralization assay \ The B95a cell was of high sensitivity for isolation of measles virus The isolated viruses analilied by RT PCR and sequence analysis were members of H1 genotype The neutralization antibody produced by measles vaccine can neutralize the wild viruses, but the titer was lower \[Conclusions\] B95a cell line can be sensitively used for measles isolation under the condition of high bio safety Measles of H1 genotype also exists in Fujian now