摘要
目的 检测神经营养素3(neurotrophin—3,NT—3)mRNA在大鼠的面肌和失神经的面肌肉的表达。方法 离断大鼠面神经的下颌缘支和颊支制备面神经损伤模型。在损伤后的第7天,采用RT—PCR技术检测NT—3mRNA在面肌和失神经的面肌内表达。结果 失神经的面肌未检测到NT—3 mRNA,有神经支配的面肌内有NT—3 mRNA的表达。结论 NT—3 mRNA在靶器官的表达可能是受其支配的神经控制。
Obejective To detect the NT-3(neurotrophin-3) mRNA expression in the
denervation and innervation facial muscles of rats. Methods The model of facial nerve le-
sion was established in rats by cutting the marginal madibular branch and buccal branches
of facial nerve. RT-PCR was employed to confirm whether NT-3 mRNA expressed in the
denervation and innervation facial muscles of rats 7 days after lesion. Results Compared
with NT-3 mRNA expression in the innervation facial muscles of rats, the NT-3. mRNA
expression were not detected in the denervation facial muscles of rats. Conclusion the
NT-3 mRNA expression in target organs was possibly controlled by neurons factor.
出处
《四川解剖学杂志》
2004年第2期95-97,共3页
Sichuan Journal of Anatomy
