摘要
休眠与萌发是植物种子对环境变化的适应特征,受许多基因调控和环境因子的影响。利用数量遗传学方法(如QTL分析)和突变等手段已对休眠和萌发特性进行了深入的遗传学研究。近些年来,随着分子生物学的快速发展,种子休眠和萌发研究已经深入到分子水平。分子生物学技术的运用,特别是基因表达、基因组测序和以双向凝胶电泳及质谱分析为技术基础的蛋白质组学分析,已成为研究种子休眠和萌发的新工具和新方向。本文主要就利用分子生物学方法研究种子休眠与萌发的进展给予简要综述。
Seed dormancy and germination are complex adaptive traits of plants influenced by a largenumber of genes and environmental factors. The use of quantitative genetics(QTL analysis)andmutant approaches has allowed further genetic studies on seed dormancy and germinationcharacteristics. Recently, following the rapid development of molecular biology, researches on thisfield are deepening into molecular level. Molecular biology techniques, especially gene expression,genome and proteome analyses based on two-dimensional gel electrophoresis and mass spectrometry,are useful approaches for the analysis of mechanism of seed dormancy and germination. This paperbriefly summaried the advances on the application of molecular biology technology in studies of seeddormancy and germination.
出处
《植物学通报》
CSCD
北大核心
2004年第2期156-163,共8页
Chinese Bulletin of Botany
基金
国家重点基础研究发展规划项目(G2000046802-05)
中国科学院知识创新工程重大项目(KSCX1-07-02)
"十五"国家科技攻关课题(2001BA606A-05-01)
中国科学院"西部之光"人才计划项目资助。