人体胆总管平滑肌细胞的组织学观察及意义

OBSERVATION OF HISTOLOGY AND CLINICAL SIGNIFICANCE OF SMOOTH MUSCLE CELL OF HUMAN COMMON BILE DUCT (CBD)

本文通过对5例正常人体胆总管(Ⅰ组)和5例胆石致扩张的胆总管(Ⅱ组)的平滑肌细胞(SMC)的组织学观察及计量分析发现:①人体胆总管(CBD)内存在着内环、外纵不成层而分散存在的SMC。②在正常人体CBD:SMC在整个肌层(?)中所占的面积分数为9.16±0.23％;在胆石致扩张的CBD组,SMC所占的面积分数为6.01±3.10％,两者在统计学上无显著差别。在Ⅱ组随着胆管壁厚度不同,SMC所占的面积分数也不一致,进行二者的相关性分析发现二者呈直线相关:y=2.7973x-2.3437,r为97.8375％。③Ⅱ组SMC的超微结构与具有明显收缩功能的胆囊颈部的SMC较相似,具有大量的细胞膜小凹和肌丝、密体等。据上述发现,我们认为人类CBD所具备的SMC具有收缩功能的结构基础,可能参与了CBD的排胆活动。

Histological studies have been Performed in 5 normal common bile ducts (CBD) (group Ⅰ). which were obtained from instant postmortem after tratffic accidents, and another 5 patients (grouo Ⅱ), which were involved in Proximal CBD dilation and terminal CBD obstruction with stones, to investigate the fine structure of smooth muscle cell (SMC) of human CBD and to assess its clinical significance. All of the sections (Ⅰ, Ⅱ) were examined by light microscope and morphometry quantitative analysis. The specimens of group Ⅱ were studied by transmisson electron microscope. Results show that the walls of the human CBD contain both longitudinal and circular smooth muscle cells. The SMC were observed in all of our studied sections. The his tological appearance of SMC contractive state is the SMC nucler torsion like a helical-structure. which was seen in two groups. Morphometry quantitative analysis indicates that the quantitative parameters (number, diameter, area, etc.) in group Ⅰ are greater than group 11 but on statistics the probability is more than 0.05 (P>0.05). The thicknness of CBD and SMC area Percent (％) is linear correlated in group Ⅱ: y=2.7973x-2.3437, r=97.8375％. The ultrastructures of the human CBD SMC (caveolas, dense area, etc.) are the same as the neck SMC of cholecyst whose contractive function is confirmed by oral choleeystography. It is suggested that the human CBD has SMC. which contains the contractile unit. theoretically, these SMC have the contractive function and may participate in the CBD movement of bile excretion.