硝普钠对豚鼠耳蜗外毛细胞能动性影响的初步研究

Influence of Sodium Nitroprusside on Cell Motility of Outer Hair Cells of Guinea Pigs

目的 研究硝普钠 (sodiumnitroprusside,SNP)对耳蜗OHC在电压刺激下的能动性的改变情况。方法 运用全细胞膜片钳电压钳技术 ,在正常细胞内外液的条件下 ,观察不同浓度SNP对电压性刺激引起OHC能动性的影响情况。结果 在SNP浓度低于 10 0 μMol L时 ,SNP对OHC能动性的影响不明显 ,但当SNP浓度高于 10 0μMol L时 ,SNP对OHC的能动性有明显的抑制作用 ,结果有统计学意义 (P <0 .0 5 )。结论 SNP对OHC的能动性有抑制作用 。

Objective To study the changes of cell motility of outer hair cells in the cochlear of guinea pigs under treatment of sodium nitroprusside(SNP). Methods By using the whole cell patch clamp recording technique in normal external and internal cell solution, the cell motility of outer hair cells under stimulation of voltage was observed in different concentration of SNP. Results Sodium nitroprusside apparently inhibited outer hair cells' motility when SNP concentration was higher than 100 μMol/L ( P <0.05).Conclusion SNP can inhibit the motility of OHCs in a dose-response manner.