金三莪抗实验性肝纤维化的超微结构研究

Ultrastructure study of JinSanE on experimental hepatic fibrosis

目的 观察金三莪抗大鼠肝纤维化的疗效及超微结构变化。方法 将 6 4只Wistar大鼠随机分为 4组 ,每组 16只 :正常组、模型组、金三莪低高剂量治疗组。除正常组外 ,其余三组用四氯化碳皮下注射诱导大鼠肝纤维化模型 ,金三莪治疗组大鼠于造模第 4周开始予以金三莪灌胃治疗 ,剂量分别为 6g/kg和 12 g/kg ,每日 1次 ,第 8周处死全部大鼠。电镜下检测肝组织 ,肝组织HE染色和VanGiseon染色 ,常规生化方法检测肝功能 ,放射免疫方法检测透明质酸(HA)。结果 经金三莪治疗后大鼠肝细胞间隙变窄 ,胶原纤维减少 ,肝窦内皮细胞窗孔增加 ,肝小叶结构趋于正常 ,纤维间隔明显变薄 (P <0 .0 1)。与模型组比较 ,金三莪治疗后大鼠血清ALT ,AST ,HA显著减低 (P <0 .0 5 )。结论 金三莪能有效地减轻肝纤维化大鼠的肝脏细胞损伤及纤维化程度。

Objective It is to observe the effect and ultrastructure change of JinSanE on hepatic fibrosis rats.Methods Sixty-four Wistar rats were randomly divided into normal group, model group, JinSanE low and high dose treatment group in which there was sixteen rats. The hepatic fibrosis was induced with CCl 4 subcutaneous injection. Four weed later, the rats in JinSanE treatment group were treated with JinSanE 6 and 12 g/kg per day respectively. At the eighth week, all rats were executed. The liver tissues were detected with electron microscope and dyed with HE and Van Giseon stainings. The hepatic function was detected with routine biochemical method and the hyalauronic acid (HA) was detected with RIA.Results The hepatocyte interstice was narrowed, the collagen fiber was reduced, the endothelial cell fenestra in hepatic sinus was increased, the structure of hepatic lobules was tended to normal and the fiber space was obviously attenuated (P<0.01). Compared with model group, the levels of serum ALT, AST and HA were markedly debased after the rats were treated with JinSanE (P<0.05).Conclusion JinSanE can effectively lighten liver cell injury and the fibrosis degree in hepatic fibrosis rats.