沙门菌增菌培养基增菌效果的探讨与分析研究

Enriching effect of enrichment medium for salmonellae

〔目的〕选择好的商品增菌培养基 ,以提高沙门菌的检出率。〔方法〕用一定数量的大肠埃希菌与不同数量种类的沙门菌混合培养 ,观察不同增菌液的增菌效果。〔结果〕目前市售的沙门增菌培养基的增菌效果差距很大 ,差的只有在 1:10状况下沙门菌呈优势生长 ,好的可达 1:1× 10 8或以上状况下仍呈优势生长。〔结论〕自配的SC和TTB增菌液增菌效果最好。

Objective To choose a better commercial enrichment medium that can improve the detecting rate of salmonellae.〔Methods〕E coli and salmonellae were cultured together with different culturing media.Then the enriching effect of each enrichment medium was determined.〔Results〕 The difference among the commercially available enrichment media was very significant.The salmonellae could grow well under the proportion varying from 1:10 to 1:10 8.〔Conclusions〕 The SC and TTB prepared by ourselves is better than other media in enriching effect.