单侧输尿管梗阻大鼠Smad3,7的表达及肾通胶囊的影响

Expression of Smad 3, 7 in Rats Renal Tissue after Unilateral Ureteral Obstruction and Effects of Shentong Capsule on Them

目的 研究大鼠单侧输尿管梗阻 (UUO)后TGF -β1,Smad3 ,7在肾组织的表达及肾通胶囊的影响。 方法 将 2 4只中16只行左侧输尿管结扎 ,另 8只行假手术 ,结扎后大鼠随机分为手术组、肾通治疗组 ,术后第 8d观察梗阻肾组织病理改变 ,纤维化程度通过测定肾间质纤维化的面积变化 ,应用免疫组织化学检测肾组织TGF -β1,Smad3 ,7的表达。 结果 手术组TGF -β1、Smad3的表达增加 ,Smad7的表达减少 ,与假手术组相比有显著差异 (P <0 0 1) ,肾通治疗组与手术组相比 ,TGF -β1、Smad3的表达下调 ,Smad7的表达上调 ,差异显著 (P <0 0 5 )。结论 肾通胶囊通过促进Smad7、抑制Smad3的表达 ,从而抑制TGF

Objective To investigate the expression of TGF-β1 and Smad 3, 7 in rats renal tissue after unilateral ureteral obstruction(UUO) and the effect of Shentong capsule on their expressions. Methods Sixteen rats underwent UUO and were devided into operation group and Shentong treatment group, additional 8 rats were shame operation group. The pathological change of renal tissues were examined by light microscopy 8 days after operation. The extent of renal fibrosis was determined by measuring the area of renal interstitial fibrosis. Immunhistochemistry was applied to detect the expression of TGF-β1 and Smad 3,7. Results In comparison with shame operation group, the expression of TGF-β1 and Smad 3 in operation group significantly increased, and the expression of Smad 7 significantly decreased(P<0 01). In comparison with operation group, in Shentong treatment group the expression of TGF-β1 and Smad 3 significantly decreased, and the expression of Smad 7 significantly increased (P<0 05). Conclusion Shentong capsule may decelarate renal interstitial fibrosis by inhibiting the over-expression of Smad 3 and promoting the expression of Smad 7 to down-regulate the expression of TGF-β1.