创伤性休克大鼠血浆AM与NO变化的相关性实验研究

A Correlation Study on Changes of Plasma Adrenomedulin and Nitric Oxide in Rats with Traumatic Shock

目的 探讨创伤性休克时大鼠血浆肾上腺髓质素 (AM)和一氧化氮 (NO)的变化特点及相关性。方法 将 40只SD大鼠分为对照组 ( 10只 )、休克未复苏组 ( 10只 )、休克复苏组 ( 10只 )和氨基胍组 ( 10只 )。对照组 10只麻醉后插管 ;3 0只SD大鼠制作创伤性休克动物模型 ,双侧股骨干砸伤后并经股动脉放血至MAP3 5～ 45mmHg。休克复苏组血压维持 3 0min ,然后回输失血和等量的林格氏液。氨基胍组在复苏时静脉注射AG60mg/kg。观察休克前后血浆AM和NO浓度的动态变化。结果 大鼠创伤性休克后 ,血浆AM和NO水平均高于对照组 ,复苏后 0 5h达到高峰 ,氨基胍组两指标变化不明显 ,AM的变化与NO的变化呈正相关。结论 提示AM与NO在创伤性休克发生发展中起重要的调节作用 。

Objective To investigate the changes of plasma adrenomedulin(AM) and nitric oxide(NO) and their correlation in rats with traumatic shock. Methods 40 Sprague-Dawley rats were randomly divided into control group(n=10), traumatic shock without resuscitation group (n=10), traumatic shock with resuscitation group (n=10) and aminoguanidine group (n=10). 10 rats of control group only received intubation after anesthesia. The other 30 rats were made animal models of traumatic shock by crashing both femur shafts and doing bloodletting to mean arterial pressure of 35～45mmHg via femoral artery. Hypotention of rats in traumatic shock with resuscitation group were maintained 30min, and then the lost blood was returned, followed by an infusion of equal volume of Ringer's solutions. Rats of aminoguanidine group were given aminoguanidine 60mg/kg body weight during resuscitation. The changes of Plasma levels of AM and NO in every group of rats were observed before and after shock. Results The plasma level of both AM and NO in the rats of traumatic shock was higher than that in the rats of control group, and reached the highest 30min after resuscitation. The level changes of plasma AM and NO in the rats of aminoguanidine group were not significant. The level change of plasma AM was positive correlation with the level change of plasma NO. Conclusions AM and NO may play an important role in the pathological process of traumatic shock. AM develops its role probably by NO.