大鼠肝脏缺血再灌注损伤早期肝亚细胞成份中氧自由基的变化

The Changes of Oxygen Free Radical of the Rat Liver Sub-cellular Organelle at the Early Stage of Ischemia-reperfusion Injury

目的 亚细胞水平上探索肝脏缺血再灌注损伤中氧自由基的变化规律。方法 测定肝脏局部缺血再灌注模型大鼠肝细胞线粒体及胞浆成份中的SOD总活力与MDA含量 ,并同步观察组织学改变。结果 肝脏局部缺血再灌注模型大鼠肝细胞线粒体及胞浆成分中的SOD总活力与MDA含量逐步升高 ,光镜及电镜下的组织学改变逐渐加重。结论 大鼠缺血再灌注的早期损伤是在再灌注一开始就发生 ,而且首先损伤线粒体 ,但被自身防御系统的作用而有所延迟。

Objective To study the change principles of the oxygen free radical on the sub-cellular level in the process of ischemia-reperfusion injury. Methods The activity of superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in the mitochondria and cytoplasm of rat liver with local ischemia-reperfusion were assayed. The histological changes of the liver were observed at the same time. Results The activity of SOD and the content of MDA kept rising and the histological changes of the liver cells kept aggravating in the process of ischemia-reperfusion. Conclusion The ischemia-reperfusion injury of rat liver occurred at the beginning of reperfusion and mitochondria was damaged first, however the injury action could be delayed by self defense.