摘要
在昆明(KM)小鼠标准化研究中,采集武汉生物制品研究所、上海第一生化制药厂普通级KM小鼠的精子并冷冻运输,冻融精子与中科院上海实验动物中心KM雌鼠的超排卵体外受精(IVF)得到杂交一代2细胞胚胎,用中科院动物中心KM鼠冻融精子对照。无菌条件下2细胞胚胎移植入SPF级假孕鼠,受体饲养于独立通气笼具(IVC)中。平均产仔率为51.9%,离乳率100%。三种群12周龄KM雄鼠精子冷冻复苏率,IVF率,移植成绩均无显著差异(P>0.05)。仔鼠经微生物、寄生虫学检测达SPF标准。结果表明,利用冷冻精子,通过IVF、胚胎移植能够方便快速的建立种群间杂交一代,并实现生物净化。
Frozen/thawed sperm, obtained from KM mice in Wu Han Institute of Biologic Products and Shanghai Biochemistry Pharmaceuticals Factory, were fertilized in vitro with superovulated oocytes from Shanghai Laboratory Animal Center KM mice. The 2-cell embryos were transplanted to SPF pseudopregnant ICR recipients. Frozen/thawed sperm from KM mice in Shanghai Laboratory Animal Center were used as control. This study showed that there were no significant difference in the thawed sperm activity rate, IVF rate and offspring produced rate among the three colonies and the results of microbiological monitoring revealed that all pups were SPF. Compared with traditional breeding method, the sperm cryopreservation and IVF technique are more efficient and convenient in standardization breeding of KM mouse.
出处
《上海实验动物科学》
2004年第1期16-18,60,共4页
Shanghai Laboratory Animal Science
基金
国家科技部基础性工作专项基金项目(2001EDA0001)
