禽白血病病毒J亚群(ALV-J)基因表达与抗原定位的研究

Studies on Gene Expression and Antigen Location of Avian Leukosis Virus Subgroup J (ALV-J)

本研究通过原位杂交(ISH)和免疫组织化学(IHC)技术检测了组织中ALV-J病毒RNA的表达及定位。原位杂交结果显示:在攻毒后3周时,所检测到的组织大部分已感染病毒;病毒对肝脏、心脏、肾脏的实质细胞、骨髓髓系细胞和卵巢基质中的间质细胞、脾脏红髓内的单核-巨噬细胞有较高的嗜性,在核膜及胞浆内显示出蓝紫色颗粒状的特异性信号,而在法氏囊、胸腺、脑和坐骨神经中检测不到病毒RNA及病毒基因的表达。免疫组化结果与原位杂交相似,在核膜及胞浆内可见蓝紫色阳性信号,瘤组织的信号较强,显示了较强的抗原性。由骨髓和其他组织的结果可推测ALV-J诱导肿瘤可能和病毒基因的插入位点有直接关系,而和病毒在组织内的数量没有直接关系。

Avian myelcytomatosis is induced by avian leukosis virus subgroup J (ALV-J) in broiler chickens. In this paper an in situ hybridization (ISH) technique and immunohitochemistry technique were used to detect RNA transcripts and locat the antigen in tissues from chickens infected with ALV-J Inner Mongolia strains (NM8761). 2 chickens were killed at 3,5,7,8,10,14,23,27 weeks respectively, different tissues were collected for further tests. The results of in situ hybridization and immunohistochemistry were identical. The greatest env expression was observed in cells specific to the tumor, liver, heart, kidney, myeloid linear cells, overy parenchymal cells. Viral gene expression could not be detected in the bursa of Fabricius, thymus, brain, ischiadic nerve in both tests. All the results indicated that myelocytes neoplasia had correlation with insertion site of ALV-J provirus in host cells genome, and had not direct correlation with the volumes of virus.