10种中药成分对CEF的增殖和抵抗NDV感染的影响

Effects of Ten Chinese Herbal Medicinal Ingredients on Proliferation and Resisting NDV Infection of CEF

分别将5种浓度的黄芪多糖(APS)、当归多糖(CAPS)、淫羊藿多糖(EPS)、板蓝根多糖(IRPS)、蜂胶多糖(PPS)、黄芪黄酮(AF)、淫羊藿黄酮(EF)、蜂胶黄酮(PF)、黄芪皂甙(AS)和人参皂甙(GS)10种中药成分加入到培养24h的鸡胚成纤维细胞(CEF)中,再培养12h后接种新城疫病毒(NDV),于病毒接种后72h用中性红染料吸收法测定CEF活性以评价各中药成分对细胞增殖及其抵抗病毒感染的影响。结果表明,10种中药成分均能不同程度地促进细胞增殖和抵抗病毒感染,前者以AF、APS、IRPS、PF和GS的作用较强,后者以GS和IRPS的作用较强,部分中药成分在两方面作用中各有所长,且有一定的量效关系。

Ten Chinese herbal medicinal ingredients, astragalus polysaccharide(APS), Chinese angelica polysaccharide(CAPS), epimedium polysaccharide(EPS), isatis root polysaccharide(IRPS), propolis polysaccharide (PPS), astragalus flavone (AF), epimedium flavone (EF), propolis flavone (PF), astragalosides (AS) and ginsenosides (GS), were added into chick embryo fibroblast (CEF), which were cultured for 24 hours, at five concentration respectively. After 12 hours, the cell was inoculated with Newcastle disease virus (NDV). At 72 hours after inoculation, the cellular activity was tested by Neutral Red Assay to evaluate the effects of the ten ingredients on proliferation and antiviral infection of the cell. The results showed that all of the ten could promote the cell proliferation and resisting the virus infection in different degrees. The better was AF,APS,IRPS,PF and GS in proliferation-promoting and GS and IRPS in antiviral infection. Some ingredients had their stronger one in two actions. There were certain dose-effect relationships in these actions.