血管紧张素原基因启动子区域单核苷酸多态性与心肌梗死的相关性

Correlation between Single Nucleotide Polymorphisms in the Promoter Region of Angiotensinogen Gene and Myocardial Infarction

为研究血管紧张素原基因启动子区域 - 2 17、- 2 0位和 - 6位上的三种单核苷酸多态性与心肌梗死的相关性 ,采用多重SnaPshot反应 ,在中国南方汉人群中 ,对 2 16例心肌梗死患者和 185名健康对照者进行G 2 17A ,A 2 0C和G 6A多态基因分型。结果发现 ,G 2 17A多态AA、AG和GG基因型分布和A、G等位基因频率在心肌梗死组与对照组之间相比有显著性差异 (分别为 10、77、12 9比 8、37、14 0 ,P =0 .0 0 2 ;2 2 .4 5 %、77.5 5 %比 14 .32 %、85 .6 8% ,P=0 .0 0 3)。G 6A多态AA、AG和GG基因型分布在心肌梗死组和对照组之间亦有显著性差异 (分别为 14 7、6 4、5比12 7、4 4、14 ,P =0 .0 2 9) ,但A、G等位基因频率在两组间无显著性差异 (P =0 .394 )。A 2 0C多态CC、AC和AA基因型分布在两组间有差异 (分别为 6、5 1、15 9比 2、6 1、12 2 ) ,但无统计学意义 (P =0 .0 6 7) ,C、A等位基因频率在两组间亦无显著性差异 (P >0 .0 5 )。Logistic回归分析发现 ,年龄 (P =0 .0 0 1)、收缩压 (P =0 .0 13)和血浆甘油三酯浓度 (P =0 .0 10 )是该人群发生心肌梗死的独立危险因素 ,而高密度脂蛋白胆固醇 (P =0 .0 18)是一种保护因素。结果提示 ,在中国南方汉人群中 ,血管紧张素原基因G 2 17A和G 6A多态可能与心肌梗死的发生?

Aim To evaluate the correlation between three single nucleotide polymorphisms (SNPs) at -217,-20 and -6 locus in the promoter region of angiotensinogen (AGT) gene and myocardial infarction (MI) in south Han Chinese population. Methods ABI PRISM SNaPshot Multiplex Kit was used to genotyping G-217A, A-20C and G-6A polymorphisms in 216 patients with MI and 185 healthy controls. Results The AA, AG and GG genotypes of G-217A polymorphism were obviously different between group of MI and group of controls (10, 77 and 129 to 8, 37 and 140, P=0.002 ). Frequencies of A and G alleles were also significantly different between two groups (22.45%, 77.55% to 14.32%, 85.68%, P=0.003). The AA, AG and GG genotypes of G-6A polymorphism were obviously different between two groups (147, 64 and 5 to 127, 44 and 14, P=0.029). But, frequencies of A and G alleles of G-6A had no significant difference between two groups (P=0.394). The CC, AC and AA genotypes of A-20C polymorphism were different between two groups (6, 51 and 159 to 2, 61 and 122), but no statistical difference was found (P=0.067). Logistic regression analysis showed that age (P=0.001), systolic blood pressure (SBP) (P=0.013) and serum concentration of triglyceride (TG) (P=0.010) were all independent risk factors for MI. Serum concentration of high density lipoprotein-cholesterol (HDLC) (P=0.018) was a protective factor for MI. Conclusions G-217A and G-6A polymorphisms of AGT gene might be associated with the occurrence of myocardial infarction in south Han Chinese population.