应激性溃疡大鼠血浆EGF水平和胃上皮细胞凋亡的变化被引量：4

Changes of Plasma EGF and Apoptosis of Gastric Epithelium Mucosae in Experimental Stress-induced Ulcers in Rats

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摘要目的　探讨应激性溃疡大鼠胃黏膜上皮细胞凋亡以及血浆表皮生长因子 (epidermalgrowthfactor,EGF)的变化。方法　制作大鼠水浸束缚应激 (water immersionandrestraintstress,WRS)模型。采用原位末端标记(TUNEL)法、电镜和荧光染色共聚焦显微镜方法分别检测胃黏膜上皮细胞的凋亡 ;放免法检测不同时段WRS大鼠血浆EGF的含量。结果　实验组与对照组相比 :血浆EGF含量明显下降 (P <0 .0 5 ) ,胃黏膜上皮细胞凋亡明显增加 (P <0 .0 5 ) ,溃疡指数 (UI)明显增加 (P <0 .0 1) ,且与应激时间呈正相关 (r =0 .975 0 ,P <0 .0 1)。结论　应激促使大鼠胃上皮细胞凋亡 ,凋亡参与了胃黏膜的损害过程 ;应激引起血浆EGF含量的下降 ;胃黏膜的损害可能与失去内源性EGF对胃黏膜的保护作用有关。 Objective To investigate the change of plasma EGF and apoptosis of gastric epithelium mucosae in experimental stress-induced ulcers in rats. Methods Forty rats were exposed to water-immersion restraint stress (WRS).The appoptotic cells in the gastric mucosa were examined through the methods of terminal deoxynucleatidyl transferase mediated DUTP nick and labeling(TUNEL),electron microscope and confocal microscopy;plasma EGF of different time points in experimental stress-induced ulcers in rats were detected by radioimmunoassay. Results Experimental group compared with those in the control group,the concentration of plasma EGF significantly decreased(P<0.05),apoptosis of gastric epithelium mucosae significantly increased(P<0.05),ulcer index significantly increased(P<0.01) and positively correlated with duration of stress (r=0.9750,P<0.01). Conclusion Stress promote apoptosis of gastric epithelium mucosae,apoptosis must participate the progress of lesion in gastric epithelium mucosae;stress cause plasma EGF decreased,the lesion may correlate with the lossed protection to gastric epithelium mucosae of endogenetic EGF.

作者徐俊宋于刚武金宝赖卓胜王亚东

机构地区第一军医大学全军消化研究所

出处《苏州大学学报（医学版）》 CAS 2004年第2期160-163,175,共5页 Suzhou University Journal of Medical Science

关键词血浆表皮生长因子溃疡应激细胞凋亡 epidermal growth factor ulcer stress apoptosis

分类号 R573.1 [医药卫生—消化系统]

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