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细胞凋亡及其检测技术的进展 被引量:5

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摘要 细胞凋亡是多细胞生物体内一个重要的生命现象 ,它已成为当前生命科学和医学领域的研究热点。根据细胞凋亡时的形态学改变和生化改变 ,通过显微镜和流式细胞仪等仪器设备 ,采用凝胶电泳及原位标记法等方法对凋亡细胞进行检测。随着研究的深入 ,许多新的指标逐渐被采纳 ,同时先进仪器的使用 ,原有指标的灵敏度和准确性也得到提高 ,这些都将细胞凋亡的检测水平提升到了一个新的高度。检测方法虽然众多 ,但多数学者认为宜选两种或两种以上的方法加以证实。
作者 赵文丽
机构地区 山西职工医学院
出处 《山西职工医学院学报》 CAS 2004年第2期68-69,共2页 Journal of Shanxi Medical College for Continuing Education
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参考文献8

  • 1[1]Martelli AM.Viruses and apopotosis[J].Cell Biochem,2001,82:634.
  • 2[2]Van Engelend M,Nieland LJ,Ramaekers FC,et al.Annexin V-affinity assay:a review on an apoptosis detection system based on phosphatidylserine exposure[J].Cytometry,1998,31(1):1-9.
  • 3[3]Hamatake M.Cell death and apoptosis[J].Biochem(TOKYO),2000,128:933.
  • 4[4]Novartis Pharma AG.Cell apoptosis[J].Toxicol Pathol,2002,30:11.
  • 5[5]Ormeerod M,Neill CF,Robertson D,et al.Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degration of DNA[J].Exp Cell Res,1994,211(2):231-237.
  • 6[6]henkart PA.Apoptosis:O death,where is thy sting[J].J Immunol,1995,154(10):4905-4908.
  • 7[7]Kouri JB,Abud-lozoya K.Arthritis R.Labelling DNA Strand breaks with BrduTP[J].Detection of apoptosis and cdll proliferation,2002,105(11):2103-2105.
  • 8[8]Gorczyca W,Gong J,Ardelt B,et al.The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents[J].Cancers,1998,53(13):3186-3192.

同被引文献65

  • 1张建海,郝俊虎,王俊东,梁琛.细胞凋亡的生理意义及其检测技术[J].中国动物检疫,2004,21(9):43-45. 被引量:3
  • 2刘伍梅,汪铭书,程安春.细胞凋亡检测技术的研究进展[J].中国兽医科技,2004,34(11):45-49. 被引量:14
  • 3高建新,周玉琴,张茹华,马雪莲,刘克敬.Caspase-3在roscovitine诱发PC12细胞凋亡中发挥重要作用[J].生理学报,2005,57(6):755-760. 被引量:5
  • 4Shin Sik Choi,Won Jong Rhee,Tai Hyun Park.Beneficial Effect of Silkworm Hemolymph on a CHO Cell System:Inhibition of Apoptosis and Increase of EPO Production[J].Biotechnology and Bioengineering,2005,9:793~800
  • 5Proskuryakov S Y,Gabai V L and Konoplyannikov A G.Necrosis is an active and controlled form of programmed cell death[J].Biochemistry (Mosc),2002,67(4):387~408.
  • 6Hershberger P A,Dickson J A and Friesen P D.Sitespecific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosos virus:Cell-line specific effects on virus replication.[J].Virol,1992,66:5525~5533.
  • 7Debbas M and White E.Wild-type p53 mediates apoptosis by E1A,which is inhibited by E1B[J].Genes Dev,1993,7:546~554.
  • 8Shizuo George Kamita,Kei Majima,Susumu Maeda*.Identification and Characterization of the p35 Gene of Bombyx moni Nuclear Polyhedrosis Virus That Prevents Virus-Induced Apoptosis[J].Journal of Virology.1993,1,455~463
  • 9Kazuhiro Okano,Toru Shimada,Kazuei Mita,Susumu Maeda.Comparative Expressed-Sequence-Tag Analysis of Differential Gene Expression Profiles in BmNPV-Infected BmN Cells[J].Virology 2001,282,348~356
  • 10Wijsman J H,Jonker R R and Keijizer R.A new method to detect apoptosis in paraffin sections:in situ endlabelling of fragmented DNA[J].J Histochem Cytochem,1993,41:7~12.

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