头孢硫脒与其他6种抗菌药物对表皮葡萄球菌体外联合药敏的研究

Antibacterial activity of cefathiamidine in vitro combined with 6 antimicrobial agents against Staphylococcus epidermidis

目的 :评价头孢硫脒分别与万古霉素、奈替米星、阿米卡星、环丙沙星、左氧沙星和加替沙星等 6种抗菌药物联合用药 ,对于表皮葡萄球菌 (Staphylo coccusepidermidis ,SE)的体外联合抗菌效应。方法 :采用棋盘法设计 ,微量肉汤稀释法测定不同浓度组合的 6组抗菌药物对 30株临床分离的表皮葡萄球菌的最低抑菌浓度 (MIC) ,并计算部分抑菌指数 (FIC指数 )。结果 :头孢硫脒对表皮葡萄球菌的MIC50 、MIC90 为 1、6 4mg·L-1;与万古霉素、奈替米星、阿米卡星、环丙沙星、左氧沙星、加替沙星联合应用后 ,其MIC50 分别降低至 0 .12 5、0 .12 5、0 .12 5、0 .12 5、0 .2 5、0 .12 5mg·L-1,MIC90 分别降低至 1、1、1、2、1、1mg·L-1。结论 :6种抗菌药物与头孢硫脒联合用药后 ,对表皮葡萄球菌球菌基本表现为协同或相加作用 ,并以协同作用为主 ,无关作用较少 ,无拮抗作用。

AIM: To evaluate the antibiotic effects in vitro of cefathiamidine combined with 6 antimicrobial agents against Staphylococcus epidermidis. METHODS: The protocol was designed by checkerboard method, the MICs against 30 strains of Staphylococcus epidermidis were determined by broth dilution method,and the FIC index was calculated according to MIC values. RESULTS: The MIC_ 50 of cefathiamidine alone against Staphylococcus aureus was 16 mg·L -1 and the MIC_ 50 of cefathiamidine combined with 6 antimicrobial agents was reduced to 0.125 , 0.125 , 0.125 , 0.125 , 0.25 , 0.125 mg·L -1 ,respectively. The MIC_ 90 of cefathiamidine alone against Staphylococcus epidermidis was reduced to 1,1,1,2, 1 mg·L -1 ,respectively. CONCLUSION: The synergism and additivity are shown when cefathiamidine combined with 6 antimicrobial agents against 30 strains Staphylococcus epidermidis,and the autonomy and antagonism are not found.