蜕皮甾酮对实验性蛛网膜下腔出血后血管内皮细胞损害的作用研究

Effects of ecdysterone on injury of endothelial cells following experimental subarachnoid hemorrhage

目的 :观察血性脑脊液 (BCSF)对培养的微血管内皮细胞 (brainmicrovesselendothelialcells ,Bm vECs)损害作用和蜕皮甾酮 (ecdysterone ,EDS)的干预效应。方法 :将培养的BmvECs分为对照组、BCSF组和EDS组 ,通过病理形态学改变、细胞计数、MTT比色法和流式细胞仪细胞周期分析评估其损害和增殖状况。结果 :与对照组比较 ,BCSF组内皮细胞出现明显的病理形态学改变 ,贴壁细胞数量减少 ,MTT吸光度下降 ,G0 ～G1期细胞比例减少。EDS对BCSF所致上述内皮细胞改变有一定的改善作用。结论 :EDS对BCSF所致的内皮形态和增殖损害具有保护作用 ,可能在慢性脑血管痉挛治疗中具有一定作用。

AIM: To investigate the effects of ecdysterone (EDS) on injury and proliferation of endothelial cells caused by bloody cerebralspinal fluid (BCSF). METHODS: Rabbit brain microvessel endothelial cells (BmvECs) were divided into control group,BCSF group and EDS group,which were treated with DMEM,BCSF and both of BCSF and EDS ( 200 mg·L -1 ),respectively. Morphological changes,cell counting,MTT assay,and flow cytometric analysis were used to evaluate the cytotoxic effects on BmvECs. RESULTS: BCSF could cause severe morphologic changes in BmvECs,decrease the OD of MTT assay and cell proportion of G_0-G_1 of BmvECs(P< 0.01 ). EDS could alleviate these changes in BmvECs caused by BCSF. CONCLUSION: EDS has protective effects on the injury of BmvECs caused by BCSF,which suggests EDS may play a role in the therapy of CVS.